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1994 (1)
1981 (1)
1Author    U. Zähringer1, E. Schaller2, H. Grisebach1Requires cookie*
 Title    Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean  
 Abstract    Using HPLC on a partisil column (5 nm) it was possible to separate two reaction products from an enzyme incubation o f dimethylallylpyrophosphate and 3,6a,9-trihydtoxypterocarpan with a particulate fraction from elicitor-treated soybean cotyledons (Harosoy 63) or soybean cell cultures. These two products were identified by MS and !H-NMR analysis as 2-and 4-dimethylallyltrihy-droxypterocarpan. Both compounds also occur as natural products in elicitor-treated soybean cotyledons and to a smaller extent in soybean cell cultures. Introduction of the dimethylallyl substituent into trihydroxypterocarpan increases the fungitoxicity against Cladosporium cucume-rinum. Upon treatment with alkali, solutions o f 3,6a,9-trihydroxypterocarpans turn to an intense red colour with Amax around 530 nm. One of the products from 2-DMA-THP could be isolated by reversed phase HPLC. According to its mass spectrum a quinoid structure is proposed for this red pigment. 
  Reference    Z. Naturforsch. 36c, 234—241 (1981); received December 81980 
  Published    1981 
  Keywords    Phytoalexins, Pterocarpans, Dimethylallyl Transferase, Soybean, Glycine max L, Red Pigment 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0234.pdf 
 Identifier    ZNC-1981-36c-0234 
 Volume    36 
2Author    SachioW. Akayam, K. Azuhito, K. Usaka, T. Sutom, K. Anehira, Y. Asuyuki, Y. Am Ada, Kazuyoshi Kawazu, A. Kio, K. ObayashiRequires cookie*
 Title    Kinobeon A, A Novel Red Pigment Produced in Safflower Tissue Culture Systems  
 Abstract    The production of safflower pigments by tissue culture techniques was carried out using the calh induced from various parts of the plant. After massive cell selection efforts, a cul­ ture cell line (KB 7) was found to produce a considerable amount of a red pigment. Addi­ tion of cellulose powder and D-phenylalanine into the medium dramatically improved the pigment production. After purification, red crystals were obtained. Its UV/VIS spectrum as well as the HPLC behavior was clearly different from that of carthamin found in the mother plant and those of another typical plant pigments, suggesting that it was a novel compound. Therefore, this pigment was named kinobeon A. 
  Reference    Z. Naturforsch. 49c, 1—5 (1994); received October 12/November 111993 
  Published    1994 
  Keywords    Kinobeon A, Red Pigment, Safflower, Carthamus tinctorius, Plant Tissue Culture 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0001.pdf 
 Identifier    ZNC-1994-49c-0001 
 Volume    49