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1988 (1)
1980 (1)
1976 (1)
1Author    Horst Pitterich, Rüdiger LawaczeckRequires cookie*
 Title    Effect of Preparative Procedures on Ghostcells from Bovine Erythrocytes  
 Abstract    Several methods for the preparation of "white ghosts" or "resealed ghosts" were described in the recent literature. This article compares three methods to prepare white, resealed ghosts from bovine erythrocytes based on the principle of hypotonic lysis. The methods described differ by the removal of hemoglobin from the empty cells. The main difference between the standard centrifu-gation, the gelfiltration and the hollow-fibre diafiltration is the mechanical stress on the leaky membranes after swelling in hypotonic media. Mean cellular volumes, rates of potassium-efflux and the access of impermeable dyes to cytoplasmatic proteins are criteria to differentiate between ghostcell-populations. 
  Reference    Z. Naturforsch. 43c, 749—753 (1988); received March 18 1988 
  Published    1988 
  Keywords    Red Blood Cells, Erythrocyte Ghosts, Gelfiltration, Diafiltration 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0749.pdf 
 Identifier    ZNC-1988-43c-0749 
 Volume    43 
2Author    U. Zimmermann, G. PilwatRequires cookie*
 Title    Organspezifische Applikation von pharmazeutisch aktiven Substanzen über zellulare Trägersysteme Organ Specific Application of Drugs by Means of Cellular Capsule Systems  
 Abstract    It is suggested to use living cells (red blood cells, lymphocytes and leucocytes) as drug delivery systems for temporal and spatial drug administration in human therapeutics and diagnosis. The effectiveness of drug loaded cells is demonstrated for the drug methotrexate which is used in cancer treatment. Red blood cells are loaded with methotrexate using the dielectric breakdown technique. Dielec­ tric breakdown leads to a transient increase of permeability of the cell membrane. Red blood cells loaded with tritium-labelled methotrexate were injected into mice and the activity level was measured in several organs as a function of time. It is shown that with this drug delivery system more than 50% of the drug (after 10 min) can be accumulated in the liver and that a high activity level can be sustained in this organ for more than 3 hours. On the other hand, administra­ tion of this drug by injecting solutions in the usual manner leads only to an 25% accumulation of methotrexate (after 10 min) in the liver. The drug is excreted completely after 1 to 2 hours. It is proposed to load red blood cells simultaneously with para-or ferromagnetic substances to obtain organ-specificity for any selected site of the body. 
  Reference    (Z. Naturforsch. 31c, 732 [1976]; eingegangen am 24. September 1976) 
  Published    1976 
  Keywords    Dielectric Breakdown, Red Blood Cells, Methotrexate, Controlled Drug Release, Organspecificity 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0732.pdf 
 Identifier    ZNC-1976-31c-0732 
 Volume    31 
3Author    Günter Pilwat, Peter Washausen, Joachim Klein, Ulrich ZimmermannRequires cookie*
 Title    Immobilization of Human Red Blood Cells  
 Abstract    H uman red blood cells were immobilized in an alginate network which was cross-linked with C a2+ ions. The im ­ mobilized cells were stored for longer than 5 weeks at 4 °C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The im ­ mobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the im mobilized and released cells retain their biconcave shape over the storage period. Measurements of the released cell popula­ tion in a hydrodynamically focussing Coulter C ounter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity have not changed in contrast to control m easurem ents on red blood cells stored conventionally in suspension indicating that im mobilization preserves cellular functions. 
  Reference    Z. Naturforsch. 35c, 352—356 (1980); received December 4 1979 
  Published    1980 
  Keywords    Red Blood Cells, Immobilization, Reversible Gels, Electri­ cal Breakdown, Coulter Counter 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0352_n.pdf 
 Identifier    ZNC-1980-35c-0352_n 
 Volume    35