| | 1 | Author
| Günter Pilwat, Peter Washausen, Joachim Klein, Ulrich Zimmermann | Requires cookie* | | | Title
| Immobilization of Human Red Blood Cells  | | | | Abstract
| H uman red blood cells were immobilized in an alginate network which was cross-linked with C a2+ ions. The im mobilized cells were stored for longer than 5 weeks at 4 °C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The im mobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the im mobilized and released cells retain their biconcave shape over the storage period. Measurements of the released cell popula tion in a hydrodynamically focussing Coulter C ounter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity have not changed in contrast to control m easurem ents on red blood cells stored conventionally in suspension indicating that im mobilization preserves cellular functions. | | | |
Reference
| Z. Naturforsch. 35c, 352—356 (1980); received December 4 1979 | | | |
Published
| 1980 | | | |
Keywords
| Red Blood Cells, Immobilization, Reversible Gels, Electri cal Breakdown, Coulter Counter | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0352_n.pdf | | | | Identifier
| ZNC-1980-35c-0352_n | | | | Volume
| 35 | |
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