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'Reconstitution' in keywords Facet   Publication Year 1981  [X]
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1981[X]
1Author    G. Ünther Bernhardt, Rainer Rudolph, Rainer JaenickeRequires cookie*
 Title    Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde  
 Abstract    Cross-linking with glutaraldehyde has been successfully applied in order to analyze the kinetics of reassociation of oligomeric enzymes (R. Hermann, R. Rudolph, and R. Jaenicke Nature 277, 243-245 (1979)). In the present study the assembly of lactic dehydrogenase from pig heart is investigated using this approach. In order to eliminate perturbations caused by excessive folding reactions, acid dissociation was performed in the presence of 0.8 M Na2S 0 4 at 0°C . Under optimum conditions complete cross-linking of the tetrameric enzyme was achieved in less than 2 minutes. Cross-linking during reconstitution proves the dimer to be the only intermediate of reasso­ ciation. The dimer -*■ tetramer transition is found to be rate-limiting for both reassociation and reactivation, suggesting the tetramer to be the enzymatically active species. The presence of monomers during reconstitution indicates that tetramer formation is preceded by a fast monomer-dimer equilibrium. The kinetic model describing the experimental data 4M^2D^T is characterized by an equilibrium constant K = 3 ± 1 • 107 liter • m ol-1, and a second-order rate constant k = 1.4 ± 0.2 • 104 liter • mol-1 • s-1. 
  Reference    Z. Naturforsch. 36c, 772—777 (1981); received May 15 1981 
  Published    1981 
  Keywords    Cross-Linking, Glutaraldehyde, Lactic Dehydrogenase, Reassociation, Reconstitution 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0772.pdf 
 Identifier    ZNC-1981-36c-0772 
 Volume    36