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'Reconstitution' in keywords Facet   Publication Year 1977  [X]
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1Author    Götz Harnischfeger, Reinhard SchopfRequires cookie*
 Title    A Fluorescence Method for Measuring the Retention of Coupling Factor (CFj) in Reconstitution Experiments of Photophosphorylation  
 Abstract    The recombination of chloroplast coupling factor 1 (CFj) and thylakoid membranes in reconsti­ tution experiments was studied through the fluorescence of paired labels covalently bound to NH,-groups. It was found that maximum recombination is achieved at a ratio of 1.5 —3,ag CF tl/ug chlorophyll. The addition of chloroplast lipids to the medium enhances the incorporation of CFt into the membranes. The rates of ATP formation of the regenerated but labeled system are decreased to 50% of those found in unlabeled control experiments. This is discussed in context with the previous observation, that labeling of CFX at similar concentrations inhibits the ATPase activity of the isolated protein completely. The possible use of double labeling in the study of the physical aspects of the reconstitution of the photophosphorylating system is discussed. 
  Reference    (Z. Naturforsch. 32c, 392 [1977]; received January 26/February 16 1977) 
  Published    1977 
  Keywords    Fluorescence Label, Coupling Factor 1, Reconstitution, Photophosphorylation 
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 TEI-XML for    default:Reihe_C/32/ZNC-1977-32c-0392.pdf 
 Identifier    ZNC-1977-32c-0392 
 Volume    32