| | 1 | Author
| Gertrude Swoboda, Wilhelm Hasselbach | Requires cookie* | | | Title
| Bile Salt Delipidation, Residual Phospholipids and Reactivation o f the Ca2+-ATPase from Sarcoplasmic Reticulum  | | | | Abstract
| 1. Delipidation o f the Ca2+-ATPase o f sarcoplasmic reticulum membranes by gel chro matography employing ionic detergents (cholate, deoxycholate and mixtures o f both) in the presence of glycerol has been studied with respect to residual phospholipids and ATPase activi ties. 2. The extent o f delipidation depends on the detergent chosen and on the ionic strength o f the elution buffer. Increasing ionic strength favours a more effective removal o f phospholipids, down to about 1 phospholipid molecule per ATPase molecule. 3. The residual ATPase activities o f the delipidated preparations are negligibly low. Extensive restoration of the Ca2+-dependent ATPase activity has been achieved by oleic acid, a lysolecithin (myristoylglycerophosphocholine) and a lecithin (dimyristoylglycerophosphocholine). The per centage of reactivation by oleate depends linearly on the amount o f residual phospholipids and on the detergent employed. 4. After gel filtration through an Ultrogel or Sepharose column containing 1% cholate in the elution buffer the delipidated ATPase is eluted as a reactivatable high molecular aggregate, whereas 1% deoxycholate favours the formation o f completely lipid-free monomeric units which cannot be reactivated, however. A high molecular aggregate is also formed in deoxycholate, the ratio of monomer to polymer depending on the solubilizing and elution conditions. 5. The residual lipids are always composed o f a mixture o f all different lipid classes present in the native sarcoplasmic vesicles, even at high degrees o f delipidation. Specific changes with vary ing extent of delipidation were not detected. | | | |
Reference
| Z. Naturforsch. 37c, 289—298 (1982); received December 7 1981 | | | |
Published
| 1982 | | | |
Keywords
| Sarcoplasmic Reticulum, Bile Salts, Delipidation, Reactivation, Lipids | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0289.pdf | | | | Identifier
| ZNC-1982-37c-0289 | | | | Volume
| 37 | |
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