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1981 (1)
1980 (2)
1979 (1)
1Author    Dieter StrackRequires cookie*
 Title    Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings  
 Abstract    Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin­ ce the addition of UDP results in a breakdown o f the ester. 
  Reference    Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0204.pdf 
 Identifier    ZNC-1980-35c-0204 
 Volume    35 
2Author    Norbert Tkotz, D. Ieter StrackRequires cookie*
 Title    from Raphanus sativus C otyledons  
 Abstract    Protein preparations from cotyledons of Raphanus sati­ vus transfer the sinapoyl moiety of 1 -sinapoylglucose to L-malate to form sinapoyl-L-malate. To our knowledge this is the first time that this type of reaction has been found to be involved in phenylpropanoid depside formation. Most acylation reactions have so far been demonstrated to depend on acyl-CoAs and only one example is known describing 1-O-acyl sugar derivatives as acyl donors. 
  Reference    Z. Naturforsch. 35c, 835—837 (1980); received June 261980 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Transacylation, Sinapoyltransferase 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0835_n.pdf 
 Identifier    ZNC-1980-35c-0835_n 
 Volume    35 
3Author    Dieter StrackRequires cookie*
 Title    Sinapine as a Supply of Choline for the Biosynthesis of Phosphatidylcholine in Raphanus sativus Seedlings  
 Abstract    Biosynthesis o f phosphatidylcholine in young seedlings o f Raphanus sativus is supplied with choline from degradation of the seed constituent sinapine (sinapoylcholine). This conclusion has been deduced from the following results: (1) Raphanus sativus seedlings accumulate approx. 70 nmol phosphatidylcholine which may be relevant for the consumption of choline, liberated during hydrolysis of approx. 130 nmol sinapine. (2) [14C]choline and [14C]ethanolamine, taken up by germinating Raphanus seeds, are exclu­ sively consumed in the biosynthesis o f phosphatidylcholine and phosphatidylethanolamine, respectively. (3) Pulse-chase experiments with seedlings at different germination stages show a reduced [14C]choline incorporation into phosphatidylcholine at the time when degradation o f sinapine occurs, obviously as a result of an isotope dilution by an increase o f the endogenous choline pool. (4) After [14C]choline pulse to immature seeds, during the process o f seed maturation most of the activity taken up is incorporated into accumulating sinapine and approx. 50% compared to this into phosphatidylcholine. During seedling development the quantity o f labelled sinapine rapidly decreases as a result of sinapine degradation with a concomitant label increase in free choline, phosphorylcholine, and phosphatidylcholine. Approx. 50% of the choline liberated from sinapine, is consumed in the biosynthesis of phosphatidylcholine. High performance liquid chromatographic analyses of phosphatidylcholine during Raphanus germination revealed that this phospholipid might be a metabolically active compound. Changes in the absorptivity of this compound at 210 nm indicate changes in the degree of unsaturation in the fatty acyl groups. 
  Reference    Z. Naturforsch. 36c, 215—221 (1981); received December 231980 
  Published    1981 
  Keywords    Sinapine, Phosphatidylcholine, Phenylpropanoids, Phospholipids, Raphanus, Brassicaceae 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0215.pdf 
 Identifier    ZNC-1981-36c-0215 
 Volume    36 
4Author    G. Erhild, N. Urm, Ann, D. Ieter StrackRequires cookie*
 Title    Sinapine Esterase I. Characterization of Sinapine Esterase from Cotyledons of Raphanus sativus  
 Abstract    From cotyledons o f Raphanus sativus (red radish) an esterase activity which catalyzes the hy­ drolysis o f sinapine into sinapic acid and choline has been isolated. The enzyme, which has a near absolute specificity, is not analogous with any esterase described in the literature. The reaction has a pH optim um o f 8.5 and the apparent K m is 1.95 x 10~5 m. The enzyme is relatively insensi­ tive to both physostigm ine (eserine) {K x = 1.73 x 10-4 m) and neostigm ine (A'i = 2 .1 3 x 10-4 m). Diisopropyl fluorophosphate (D F P) showed no inhibition and diethyl /?-nitrophenylphosphate (E 600) only a slight inhibitory effect at 10-5 m, respectively. Choline (10~2 m) was inhibitory but acetylcholine (1 0 -2 m) stimulated the enzyme activity. 
  Reference    Z. Naturforsch. 34c, 715—720 (1979); received June 11 1979 
  Published    1979 
  Keywords    Esterase, Sinapine, Sinapic Acid Esters, Raphanus, Brassicaceae, High-Performance Liquid Chro­ matography (HPLC) 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0715.pdf 
 Identifier    ZNC-1979-34c-0715 
 Volume    34