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'Raphanus' in keywords Facet   Publication Year 1980  [X]
Facet   section ZfN Section C  [X]
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1Author    Dieter StrackRequires cookie*
 Title    Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings  
 Abstract    Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin­ ce the addition of UDP results in a breakdown o f the ester. 
  Reference    Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0204.pdf 
 Identifier    ZNC-1980-35c-0204 
 Volume    35 
2Author    Norbert Tkotz, D. Ieter StrackRequires cookie*
 Title    from Raphanus sativus C otyledons  
 Abstract    Protein preparations from cotyledons of Raphanus sati­ vus transfer the sinapoyl moiety of 1 -sinapoylglucose to L-malate to form sinapoyl-L-malate. To our knowledge this is the first time that this type of reaction has been found to be involved in phenylpropanoid depside formation. Most acylation reactions have so far been demonstrated to depend on acyl-CoAs and only one example is known describing 1-O-acyl sugar derivatives as acyl donors. 
  Reference    Z. Naturforsch. 35c, 835—837 (1980); received June 261980 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Transacylation, Sinapoyltransferase 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0835_n.pdf 
 Identifier    ZNC-1980-35c-0835_n 
 Volume    35