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1996 (1)
1Author    Karlheinz TempelRequires cookie*
 Title    Radioadapted Chicken Embryo Cells: Challenge Specificity and Alterations in Higher-Order D N A Structure  
 Abstract    Radioadapted chicken embryo cells (X-irradiation in ovo with 10 cGy at the 14th day of developm ent with priming periods of 24 h) were treated in vitro by challenge doses of 14 different D N A -and/or chromatin-interactive agents, including X-rays. A decrease in the cel­ lular damage, as measured by scheduled D N A synthesis, was only observed with X-irradia­ tion. Sedimentation of nucleoids as well as viscosity of alkaline lysates from ethidium bro­ mide (0 .3 5 -4 0 0 (ig/ml)-, novobiocin (1 2 5 -1 8 0 0 ^g/ml)-, and hyperthermia (30 min at 43° and 45°)-treated cells suggest a higher tendency of radioadapted cells to undergo positive D N A supercoiling. When D N A from adapted and non-adapted chicken embryo cells was used as substrate, neither its digestion by D N ase I nor the inhibition of DNase I activity by various DNA-interactive agents was changed in primed cells. From the previous investigations as well as from the present results it is concluded that an increase of tightening of protein-DNA interactions within the nuclear matrix is a molecular determinant of the elevated radiation resistance in radioadapted chicken embryo cells. 
  Reference    Z. Naturforsch. 51c, 211 (1996); received N ovem ber 29 1995/January 5 1996 
  Published    1996 
  Keywords    Chicken Embryo, Radioadaptation, Challenge Specificity, Nucleoids 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0211.pdf 
 Identifier    ZNC-1996-51c-0211 
 Volume    51