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'RNA polymerases' in keywords Facet   section ZfN Section C  [X]
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1982 (1)
1979 (1)
1Author    ArminH. Ildebrandt, Rudolf Mengel, HelmutW. SauerRequires cookie*
 Title    Characterization of an Endogenous Transcription Inhibitor from Physarum polycephalum  
 Abstract    A substance has been purified from isolated nuclei of Physarum polycephalum by equilibrium and velocity gradient centrifugations, ion exchange chromatography and gel filtration which has a high molecular weight, can be labeled in vivo with 32P, is heat stable and resistant to amylases, proteases, nucleases and phosphodiesterase but is sensitive to phosphatases or hydrolysis. This material consists of phospate and glycerol. It selectively inhibits in vitro transcription of RNA polymerases, predominantly the homologous enzyme A by binding to the enzyme. In the presence of this inhibitor of transcription a stable RNA polymerase-template complex cannot be formed. Binding to and inactivation of RNA polymerase is reversible at high ionic strength. 
  Reference    Z. Naturforsch. 34c, 76 (1979); received July 3/ November 21 1978 
  Published    1979 
  Keywords    RNA polymerases, Inhibitor, Glycerophosphate, Physarum 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0076.pdf 
 Identifier    ZNC-1979-34c-0076 
 Volume    34 
2Author    K. Grossmann, H. Bisswanger, H. U. SeitzRequires cookie*
 Title    Differential Fluorescence and Kinetic Studies on the Template-Binding of RNA Polymerase from Parsley and Escherichia coli  
 Abstract    A fluorescence spectroscopy m ethod is described for studying association o f RNA polymerase with DNA templates. Using double beam differential fluorescence at excitation and emission wavelengths of 285 and 335 nm, respectively, the new technique discriminates non-specific decrease of fluorescence intensity by addition o f D N A from quenching of polymerase fluorescence by protein-nucleic acid interactions. C om paring the results with studies of UM P incorporation into RNA, the Äg-values o f tem plate-binding were in good agreem ent with the values for RNA synthesis, pointing to specific interaction o f polym erase and the D NA tem plate measured by the fluorescence method. W hile E. coli enzyme showed higher affinity for tem plates such as heat-denatured poly [d(A-T)] and poly [d(G -C)] parsley RNA polym erase I accepted such templates with the same affinity as salmon sperm DNA. It is obvious that divalent cations are not necessary for the interaction of both enzymes with single-stranded DNA -tem plates. UM P incorporation studies suggest that transcription is a cooperative process. 
  Reference    Z. Naturforsch. 37c, 81—86 (1982); received O ctober 261981 
  Published    1982 
  Keywords    RNA Polymerase, Template-Binding, D ifferential Fluorescence, Parsley, E coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0081.pdf 
 Identifier    ZNC-1982-37c-0081 
 Volume    37