| | 1 | Author
| M.-L Heskamp, W. Barz | Requires cookie* | | | Title
| Characterization of Proteases from Rhizopus Species after Growth on Soybean Protein  | | | | Abstract
| Culture filtrates of different fungi of the genus R hizopus forming tem pe (i.e. traditional Indonesian food) were grown on a soybean protein-raffinose-phytate medium and investi gated for protease activity using soyprotein as substrate. A ll isolates belonging to the species R. oryzae, R. stolonifer, R. oligosporus, and R. m icrosporus var. chinensis, formed the well-known R hizopus-pepsin (aspartic proteinase, 35 kD, isoelectric points: 5.9, 5.0, <4) and an additional protease mainly active under alkaline conditions. The new protease (33 kD, iso electric points: variable and isolate specific) was purified approximately 300-fold and shown to be a serine protease (inhibitor studies). During fungal culture (1 2 -1 3 5 h) the aspartic proteinase is expressed first followed by the serine protease. Both proteases are insensitive to the soybean Kunitz and Bowman-Birk inhibitors. The best rate of soyprotein degradation is achieved by the coordinate action of both proteases at pH 6.5. The examined R hizopus isolates differ in the time course and intensity o f the expression o f the alkaline protease. | | | |
Reference
| Z. Naturforsch. 52c, 595 (1997); received May 6/July 3 1997 | | | |
Published
| 1997 | | | |
Keywords
| Soyprotein, R hizopus, Proteases, Tempe, Inhibitors | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/52/ZNC-1997-52c-0595.pdf | | | | Identifier
| ZNC-1997-52c-0595 | | | | Volume
| 52 | |
|
