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1980 (1)
1978 (1)
1Author    Yoriko Taketo, Akira TaketoRequires cookie*
 Title    Effects of Polynucleotides on Production and Activity of Streptolysin S  
 Abstract    Activity o f various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact m olecules o f M S2 R N A , E. coli tR N A or rat liver R N A were almost inactive, whereas R N ase I core o f these R N A s, especially the core frac­ tions eluted from DEA E-cellulose colum n at higher NaC l concentrations, effectively induced pro­ duction o f the extracellular hem olysin. The carrier activity o f yeast R N A was significantly en­ hanced by sim ultaneous addition o f R N ase I, to the streptococcal suspension. Preincubation o f yeast R N A with growing streptococci or a protein fraction from the bacterial culture supernatant increased its carrier activity. Evidences were obtained suggesting involvem ent o f streptococcal nuclease in the enhancement o f the carrier effect. Production o f the streptococcal hem olysin was markedly prom oted by polyguanylic acid but neither by polyadenylic acid, polycytidylic acid nor by polyuridylic acid. Like trypan blue, polyguanylic acid exerted potent inhibitory effect on hem olytic activity o f streptolysin S complex. Similar but less marked effect was observed with certain R N ase I core fractions o f rat liver R N A. Som e data concerning effect o f polynucleotides on stability o f the hem olysin were also presented. 
  Reference    Z. Naturforsch. 35c, 578 (1980); received March 10 1980 
  Published    1980 
  Keywords    Streptolysin S, R N A, Polyguanylic Acid, Streptococcus pyogenes 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0578.pdf 
 Identifier    ZNC-1980-35c-0578 
 Volume    35 
2Author    J.R 0 Dawson, P. E. Dickerson, JanetM. King, F. Sakai, A.R H Trim, J. W. WattsRequires cookie*
 Title    Improved Methods for Infection of Plant Protoplasts with Viral Ribonucleic Acid  
 Abstract    Infection of tobacco protoplasts with R N A of tobacco mosaic virus and the bromoviruses has been studied. The quality of the R N A was very important. Ribonuclease activity was controlled by incorporating 5 mM ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus R N A but infection with T M V R N A was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral R N A examined. 
  Reference    Z. Naturforsch. 33c, 548—551 (1978); received May 10 1978 
  Published    1978 
  Keywords    Protoplasts, R N A, Plant Viruses, Tobacco M osaic Virus, Polyethylene Glycol 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0548.pdf 
 Identifier    ZNC-1978-33c-0548 
 Volume    33