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'Quinolizidine Alkaloids' in keywords Facet   Publication Year 1980  [X]
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1Author    M. Wink, T. Hartmann, L. WitteRequires cookie*
 Title    Enzymatic Synthesis of Quinolizidine Alkaloids in Lupin Chloroplasts  
 Abstract    The enzymatic sequence responsible for the biosynthesis o f tetracyclic quinolizidine alkaloids could be localized in chloroplasts isolated from Lupinus polyphyllus leaves and L. albus seedlings by differential centrifugation. U pon feeding of cadaverine to isolated chloroplasts lupanine is produced as the m ain alkaloid. Chloroplasts treated with digitonine produce sparteine and 17-oxosparteine instead o f lupanine, thus indicating that the biosynthetic sequence is interrupted. The intermediacy of 17-oxosparteine could be confirmed since exogeneous 17-oxosparteine is con­ verted into lupanine by intact chloroplasts. 17-Oxosparteine synthase (see Z. N aturforsch. 34 c, 704 1979) the key enzyme of quinolizidine alkaloid biosynthesis could be solubilized from chloro­ plasts treated with detergents or osmotic shock. 
  Reference    Z. Naturforsch. 35c, 93—9 (1980); received Septem ber 26 1979 
  Published    1980 
  Keywords    Quinolizidine Alkaloids, Enzymatic Synthesis, Isolated Chloroplasts, Lupinus polyphyllus 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0093.pdf 
 Identifier    ZNC-1980-35c-0093 
 Volume    35