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1	Author	K. H. Trautmann, A. Schuler, Dr Maag, Ag Dielsdorf, SchweizM. Suchý, H.-K Wipf	Requires cookie*
	Title	Eine
	Abstract	Methode zur qualitativen und quantitativen Bestimmung von drei Juvenilhormonen von Insekten Nachweis von 10.1 l-Epoxy-3.7.1 l-trimethyl-2-frans-6-//-ons-dodecadiensäuremethyl-ester in Melolontha melolontha A Method for the Qualitative and Quantitative Determination of Three Natural Insect Juvenile Hormones Evidence of Methyl 10,ll-epoxy-3,7,ll-trimethyl-2-/rans-6-*ran.s-dodecadienoate in Melolontha melolontha A method is presented permitting the qualitative and quantitative determination of all three presently known hormones (J H 1 —3). The determination is based on the method of radioactive isotope dilution, whereby a very small known amount of tritium-labelled JH-1 is added to the ether extract of the particular species. The addition of radioactive JH-1 permits the isolation of all three hormones, because of their similar behaviour during the chosen work up. The quantitative determination was carried out by gas chromatography and the identification was confirmed with the help of retention-times and GC-MS combination. The method was checked by using an extract of Hyalophora cecropia. For the first time methyl 10,ll-epoxy-3,7,ll-trimethyl-2-frans-6-/rans-dodecadienoate (JH-3) could also be identified as the juvenile hormone of Melo lontha melolontha. In Vanessa io larvae, Tenebrio molitor larvae and adults and in Musca domestica larvae none of the three known hormones could be detected. The preparation of JH-1 labelled with tritium in the methyl group of the ester was accomplished with very high specific activity (4.34 Ci/mmol) of the tritiated acid with diazomethane.
	Reference	(Z. Naturforsch. 29c, 161—168 [1974]; eingegangen am 12. Dezember 1973)
	Published	1974
	Keywords	Quantitative Determination, Insects, Juvenile Hormones
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	TEI-XML for	default:Reihe_C/29/ZNC-1974-29c-0161.pdf
	Identifier	ZNC-1974-29c-0161
	Volume	29

2	Author	L. W. Van Broekhoven, A. C. Van Der Kerk, -Van Hoof, C. A. Salemink	Requires cookie*
	Title	Gas Chromatographie Determination of Subnanogram Amounts of the Juvenile Hormone Methyl (2E, 6E)-10,ll-epoxy-3,7,ll-tri- methyl-2,6-dodecadienoate (JH III) in Insect Material by Electron Capture Detection
	Abstract	Using an electron capture detector, a sensitive gas chromatographic method has been developed for the determination of the juvenile hormone methyl(2E, 6E)-lOjll-epoxy-SJjll-trimethyl-^ö-dodecadienoate (JH III) in insect material. The sensitivity in electron capture detection of the bisheptafluorobutyrate was determined. The work-up procedure was checked by adding known amounts of JH III to insect material. The method seems equally applicable to all three juvenile hormones.
	Reference	(Z. Naturforsch. 30c, 726—729 [1975]; received May 28 1975)
	Published	1975
	Keywords	Quantitative Determination, Electron Capture Detection, Insects, Juvenile Hormone
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	TEI-XML for	default:Reihe_C/30/ZNC-1975-30c-0726.pdf
	Identifier	ZNC-1975-30c-0726
	Volume	30