| | 1 | Author
| Jörg-Peter Schnitzler, HannsUlrich Seitz | Requires cookie* | | | Title
| Rapid Responses of Cultured Carrot Cells and Protoplasts to an Elicitor from the Cell Wall of Pythium aphanidermatum (Edson) Fitzp  | | | | Abstract
| , A llgem eine Botanik und P flanzenphysiologie, A u f der M orgenstelle Suspension cultured cells of an anthocyanin-containing cell line (D C b) from Daucus carota L. ssp. sativus respond very rapidly to treatment with a soluble carbohydrate elicitor from the wall of the oom ycete Pythium aphanidermatum by synthesizing 4-hydroxybenzoic acid which is incorpo rated into the plant cell wall. Enzym es of phenol m etabolism , phenylalanine am m onia-lyase (P A L) and chalcone synthase (C H S), respond to elicitor treatment in different ways. Both the catalytic activity o f PAL and its subunit concentration, measured by means o f im m unoblotting, show a transient increase upon elicitation, whereas CHS the initial enzym e o f the flavonoid pathway, is inhibited after administration of the elicitor. and consequently anthocyanin accum ula tion ceases. In protoplasts derived from the cultured cells a very similar elicitor-induced response has been observed. Lacking a cell wall, the protoplasts secrete the 4-hydroxybenzoic acid into the culture fluid. The carrot protoplasts isolated by wall degrading enzym es retain their responsive ness to the fungal carbohydrate elicitor. The value o f the protoplast system for studying the im m ediate events following elicitor treatment is discussed. | | | |
Reference
| Z. Naturforsch. 44c, 1020—1028 (1989); received Septem ber 13 1989 | | | |
Published
| 1989 | | | |
Keywords
| Carrot Cell Cultures, Elicitor, 4-Hydroxybenzoic A cid, Protoplasts, Pythium aphanidermatum | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/44/ZNC-1989-44c-1020.pdf | | | | Identifier
| ZNC-1989-44c-1020 | | | | Volume
| 44 | |
|
