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1979 (2)
1Author    Giuseppe Colacicco, A. Purba, K. Ray, MukulK. Basu, M. Urray, W. Ittner, RobertM. RosenbaumRequires cookie*
 Title    Cultured Lung Cells: Interplay Effects of Beta-Mimetics, Prostaglandins and Corticosteroids in the Biosynthesis of Dipalmitoyl Lecithin  
 Abstract    Cell lines derived from type II lung cells were used to study interplays of substances affecting incorporation of labeled precursors [1-14C] palmitate and [methyl-3H] choline into phosphatidyl choline. Ethanol stimulated markedly biosynthesis of dipalmitoyl phosphatidyl choline in cloned rabbit lung cells; the stimulating action of ethanol was reduced very much by cortisol and less by ritodrine. In the presence of 0.1 m m isoproterenol, two prostaglandins, E2 and F2a, caused marked depressions in the incorporation of both precursors by cell line A 549 derived from human lung adenocarcinoma. One concluded that among the agents studied, ethanol and cortisol are potent antagonists, and so were also the prostaglandins and isoproterenol. 
  Reference    Z. Naturforsch. 34c, 101 (1979); received October 6 1978 
  Published    1979 
  Keywords    Ethanol Metabolism, Beta-Stimulants, Lung Type II Cells, Pulmonary Surfactant, Cortisol 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0101.pdf 
 Identifier    ZNC-1979-34c-0101 
 Volume    34 
2Author    Giuseppe Colacicco, MukulK. Basu, ApurbaK. Ray, M. Urray, W. Ittner, RobertM. RosenbaumRequires cookie*
 Title    Cultured Lung Cells: Effects of Isotopic Dilution and Some Undefined Stimulation on the Incorporation of Radiolabeled Palmitate and Choline into Phosphatidyl Choline (Lecithin)  
 Abstract    Using radiolabels, we studied the effect of certain experimental conditions on the incorporation of choline and palmitate into phosphatidyl choline (lecithin) of cloned type II rat lung cells. When the label was changed from methyl-3H to 1,2-14C the incorporation of choline was reduced to 1/3; in contrast, when the label was moved from 1-14C to 9,10-3H, the incorporation of free pal­ mitate was more than doubled. Removal of choline from the culture medium caused trebling of choline incorporation and appreciable decrease in palmitate uptake, indicating respectively an expected effect of increased choline label concentration in the absence of carrier, and a marked dependence of palmitate on choline incorporation. Removal of fetal calf serum produced more than 2/3 decrease in palmitate incorporation (instead of an expected increase because of palmitate isotope concentration), whereas choline uptake was not affected, meaning respectively that either serum hormones or serum lipids, or both simultaneously, are important for palmitate but not for choline incorporation. This is only the beginning of a host of studies required to clarify the role of fetal calf serum constituents onto lecithin biosynthesis in cultured lung cells and finally gain a full appreciation of the biosynthetic pathways of phosphatidyl choline ("surfactant") in type II cells of alveolar epithelium. 
  Reference    Z. Naturforsch. 34c, 96 (1979); received September 14 1978 
  Published    1979 
  Keywords    Pulmonary Surfactant, Type II Cells, Biosynthetic Pathways, Isotopic Dilution, Serum Hormones 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0096.pdf 
 Identifier    ZNC-1979-34c-0096 
 Volume    34