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'Protoplast' in keywords Facet   Publication Year 1989  [X]
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1989[X]
1Author    Jörg-Peter Schnitzler, HannsUlrich SeitzRequires cookie*
 Title    Rapid Responses of Cultured Carrot Cells and Protoplasts to an Elicitor from the Cell Wall of Pythium aphanidermatum (Edson) Fitzp  
 Abstract    , A llgem eine Botanik und P flanzenphysiologie, A u f der M orgenstelle Suspension cultured cells of an anthocyanin-containing cell line (D C b) from Daucus carota L. ssp. sativus respond very rapidly to treatment with a soluble carbohydrate elicitor from the wall of the oom ycete Pythium aphanidermatum by synthesizing 4-hydroxybenzoic acid which is incorpo­ rated into the plant cell wall. Enzym es of phenol m etabolism , phenylalanine am m onia-lyase (P A L) and chalcone synthase (C H S), respond to elicitor treatment in different ways. Both the catalytic activity o f PAL and its subunit concentration, measured by means o f im m unoblotting, show a transient increase upon elicitation, whereas CHS the initial enzym e o f the flavonoid pathway, is inhibited after administration of the elicitor. and consequently anthocyanin accum ula­ tion ceases. In protoplasts derived from the cultured cells a very similar elicitor-induced response has been observed. Lacking a cell wall, the protoplasts secrete the 4-hydroxybenzoic acid into the culture fluid. The carrot protoplasts isolated by wall degrading enzym es retain their responsive­ ness to the fungal carbohydrate elicitor. The value o f the protoplast system for studying the im m ediate events following elicitor treatment is discussed. 
  Reference    Z. Naturforsch. 44c, 1020—1028 (1989); received Septem ber 13 1989 
  Published    1989 
  Keywords    Carrot Cell Cultures, Elicitor, 4-Hydroxybenzoic A cid, Protoplasts, Pythium aphanidermatum 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-1020.pdf 
 Identifier    ZNC-1989-44c-1020 
 Volume    44