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'Protoplast' in keywords Facet   Publication Year 1985  [X]
Facet   section ZfN Section C  [X]
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1Author    J.M F G Aerts, A. W. SchramRequires cookie*
 Title    Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals I. A Comparison of Isolation Procedures  
 Abstract    Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. U tilizing the vacuolar localization o f anthocyanin, vacuolar prep­ arations obtained through polybase treatment, osm otic shock and m echanical forces were com ­ pared on purity, yield and stability. The com parison indicated that application o f the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which m ain­ tained their acidic pH and retained their anthocyanin content. 
  Reference    Z. Naturforsch. 40c, 189—1 (1985); received June 26/O ctober 26 1984 
  Published    1985 
  Keywords    Petunia hybrida, Vacuoles, Protoplasts, Anthocyanins 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0189.pdf 
 Identifier    ZNC-1985-40c-0189 
 Volume    40 
2Author    Natalia Stoicheva, Iana Tsoneva, DimiterS. DimitrovRequires cookie*
 Title    Protoplast Dielectrophoresis in Axisymmetric Fields  
 Abstract    Pea protoplasts dielectrophoretic coefficients were measured in alternating electric fields of frequency 1 M Hz and voltage 5 V applied between two concentric cylindrical metal electrodes of outer and inner radii 0.24 mm and 1 mm, respectively. They do not vary significantly with solution osmolarity, but* show a clear expressed maximum in isotonic conditions; the values in 0.4 m , 0.5 m , 0.6 m and 0.7 m manitol solutions are (6.5, 10.2, 8.8 and 5.6) x lO * 24 A 2s4/kg, respectively. The average cell radii in those conditions are 14.2 (im, 14.6 [im, 13.8 jim and 13.6 [im. The radii of cell-to-electrode contacts follow the same dependence on the osmolarity as the dielectrophoretic coefficients; they do not depend on the applied voltages up to 18V. The times of cell approach near to the electrode were too short to be explained by the action only of the dielectrophoretic force; consequently an attractive force appeared at very close approach. These results may be of use in future studies of membrane adhesion and fusion. 
  Reference    Z. Naturforsch. 40c, 735 (1985); received February 4 1985 
  Published    1985 
  Keywords    Protoplasts, Dielectrophoresis, Axisymmetric Fields 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0735.pdf 
 Identifier    ZNC-1985-40c-0735 
 Volume    40