| | 1 | Author
| H. Janzen, E. Matuszak, E. V. Goldammer, AnnenBundesrepublik Deutschland, H. R. Wenzel | Requires cookie* | | | Title
| Thermodynamic and Magnetic Resonance Studies on the Hydration of Polymers: II. Protein-Water Interactions in Powdered Ribonuclease  | | | | Abstract
| ESR studies on spin-labeled amorphous RNase A as a function of varying concentrations of sorbed H 2 0 and D 2 0 will be presented. A relaxation analysis of saturation transfer (ST-)ESR spectra of l4 N('H) nitroxide spin-label molecules essentially fixed at amino acid residue His-105 will be given. A characteristic correlation has been observed between the microdynamic behavior — express-ed by the rotational correlation times of the paramagnetic label — and the macroscopic thermo-dynamic entropy for the sorption process of H 2 0 and D 2 0 at RNase. This correlation is particu-larly pronounced at low water concentrations, viz., n H20 /n protein < 100. A significant difference in this concentration range exists between the two systems "RNase-H 2 0" and "RNase-D 2 0", which is manifested not only by the thermodynamic data but also by the microdynamic behavior ex-tracted from the corresponding non-linear ESR absorption line shapes. | | | |
Reference
| Z. Naturforsch. 43c, 285—293 (1988); received December 18 1987 | | | |
Published
| 1988 | | | |
Keywords
| Protein, Structure, Hydration, NMR, Thermodynamic | | | |
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| default:Reihe_C/43/ZNC-1988-43c-0285.pdf | | | | Identifier
| ZNC-1988-43c-0285 | | | | Volume
| 43 | |
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