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'Protein Phosphorylation' in keywords Facet   Publication Year 1984  [X]
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1984[X]
1Author    N. Pucheu, G.F W IldnerRequires cookie*
 Title    Isolation of 3 2 -3 5 kDa Thylakoid Proteins from Chlamydomonas reinhardii  
 Abstract    The protein com position o f Chlamydomonas reinhardii thylakoids in the m olecular weight range o f 3 2 -3 5 kDa was studied. The thylakoids were labelled with 32P —Pj in vivo using Pr starved cell cultures, solubilized with SDS and separated by polyacrylam ide gradient gel electrophoresis. The following differentiation o f proteins could be accom plished: two proteins were phosphorylated, which were also well stained with C oom assie blue, and one major protein was only detected by the silver staining procedure. The m obility o f the latter protein is different in gels with urea, showing an apparently lower molecular weight. In order to investigate whether a functional photosystem II is obligatory for protein phos­ phorylation, the phosphorylation o f thylakoid proteins was studied with a photosystem II deficient mutant. The mutant, which had normal photosystem I activity, but lacked photo­ system II activity, could synthesize ATP light dependently; its m ain labelled protein bands had a molecular weight o f 3 2 -3 5 kDa; it contained the light harvesting protein chlorophyll com plex and an unknown protein at 22 kDa. The 32P incorporation in photosystem II deficient cells was comparable to cells with functional photosystem II units. 
  Reference    Z. Naturforsch. 39c, 434 (1984); received N ovem ber 30 1983 
  Published    1984 
  Keywords    Photosystem II Complex, Q B-Protein, Chlamydomonas reinhardii, Protein Phosphorylation, Photosystem II Deficiency 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0434.pdf 
 Identifier    ZNC-1984-39c-0434 
 Volume    39