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1996 (1)
1Author    M. Auro, Sola-Pennaa, JoseR. Oberto, M. Eyer-FernandesbRequires cookie*
 Title    Trehalose Protects Yeast Pyrophosphatase against Structural and Functional Damage Induced by Guanidinium Chloride  
 Abstract    Trehalose is accumulated at very high concentrations in yeasts when this organism is sub­ mitted to a stress condition. This report approaches the question on the protective effect of trehalose and its degradation product, glucose, against structural and functional damage promoted by guanidinium on yeast cytosolic pyrophosphatase. Here it is shown that both, 1 m trehalose or 2 m glucose, are able to attenuate at almost the same extent the conforma­ tional changes promoted by guanidinium chloride on the pyrophosphatase structure. On the other hand, while 1 m trehalose increases 3.8 times the K x (from 0.15 to 0.57 m) for guanidi­ nium chloride inhibition of pyrophosphatase activity, 2 m glucose did not even duplicate this parameter (from 0.15 to 0.25 m). These data support evidences for a functional reason for the accumulation by yeasts of trehalose, and not other compound, during stress conditions. 
  Reference    Z. Naturforsch. 51c, 160—164 (1996); received September 15/November 15 1995 
  Published    1996 
  Keywords    Trehalose, Yeast, Carbohydrate, Protection, Guanidinium Chloride 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0160.pdf 
 Identifier    ZNC-1996-51c-0160 
 Volume    51