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1990 (1)
1989 (1)
1Author    Stefan Stevanovic, Günther Jung, JürgP. RosenbuschRequires cookie*
 Title      
 Abstract    The major outer membrane protein of Rhodobacter capsulatus 37b4 (capsule-free) was iso­ lated. Strong porin-activity was observed after reconstitution into artificial lipid bilayer mem­ branes with a single channel conductance of 3.15 nS in Im KC1. The porin migrated as a broad, single band (M T above 90,000) on sodium dodecyl sulfate polyacrylamide gel electro­ phoresis and dissociated into a single species of polypeptides (M r 36,000) on treatment with EDTA (10 m M at 30 °C, 20 min) or by heating (100 °C, 5 min). Analytical ultracentrifugation studies demonstrated the native porin to be a trimer. The monomers chromatofocused as a single, sharp peak on fast performance liquid chromatography and only one band, corre­ sponding to an isoelectric point of about 4.0, was obtained on isoelectric focusing. Gas-phase sequencing of the 23 N-terminal residues revealed G lu-V al-Lys-Leu-Ser-G ly-A sp-Ala-Arg-M et-G ly-V al-M et-T yr-A sn-Gly-A sp-Asp-X-A sn-Phe-Ser-Ser. 
  Reference    Z. Naturforsch. 45c, 576—582 (1990); received February 19 1990 
  Published    1990 
  Keywords    Rhodobacter capsulatus, Cell Wall, Outer Membrane, Porin, Oligomeric Structure 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0576.pdf 
 Identifier    ZNC-1990-45c-0576 
 Volume    45 
2Author    UweJ. Jürgens, Roland BenzRequires cookie*
 Title    Pore-Forming Activity of Outer Membrane Extracts from the Unicellular Cyanobacterium Synechocystis sp. PCC 6714  
 Abstract    Cell walls of the unicellular cyanobacterium Synechocystis sp. PCC 6714, isolated from cell homogenates, were found to be unusually resistant against extraction with various detergents, organic solvents, chaotropic agents, and proteases. The major outer membrane proteins (M r 67,000; 61,000; 94,000) were solubilized by differential SDS-extraction and purified by prepara­ tive SDS-PAGE. The extracted proteins, reconstituted into lipid bilayer membranes, formed two types of pores with single-channel conductances of 2.2 nS (pore diameter of 1.4 nm) and 0.3 nS (pore diameter not determined), respectively. Carotenoids and lipopolysaccharide were found to be associated with the extracted major proteins. 
  Reference    Z. Naturforsch. 44c, 165—169 (1989); received October 10 1988 
  Published    1989 
  Keywords    Carotenoid-Binding Protein, Cyanobacterium, Outer Membrane Protein, Porin, Protease-Resist­ ance, Synechocystis 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0165.pdf 
 Identifier    ZNC-1989-44c-0165 
 Volume    44