| | 1 | Author
| B. Ernd, F. Ussm, Ann, P. Ete, D. An | Requires cookie* | | | Title
| Polymerization of Actin in the Absence and Presence of Cytochalasin B: Problems of Determining "Critical Concentration"  | | | | Abstract
| A Various concentrations o f actin (0.3 or 1 mM MgCl2, 1 mM A T P , 1 mM E G T A) reached their final degree o f polym erization (m easured by a pyrene dye attached to actin) earlier in the pres ence o f cytochalasin B than in its absence. The curves relating concentrations o f polym eric F-actin to total actin concentration were under these conditions highly nonlinear making an unam biguous extrapolation to zero F-actin concentration (to deduce the "critical concentration" o f actin poly m erization) im possible. The concentration o f actin, above which polym erization occurred, was unaltered by cytochalasin B (although for reasons not yet understood the specific fluorescence intensity of polym erized actin was lower in the presence of cytochalasin B than in its absence). The results show that a distinct "critical concentration" o f actin polymerization must not always be well defined. | | | |
Reference
| Z. Naturforsch. 41c, 781 (1986); received April 23 1986 | | | |
Published
| 1986 | | | |
Keywords
| ctin, Cytochalasin B, Critical Concentration, Fluorescence, Polymerization | | | |
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| default:Reihe_C/41/ZNC-1986-41c-0781.pdf | | | | Identifier
| ZNC-1986-41c-0781 | | | | Volume
| 41 | |
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