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'Polymerase Chain Reaction' in keywords
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1991 (1)
1990 (1)
1989 (1)
1Author    Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V RRequires cookie*
 Title    A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  
 Abstract    Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel­ ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi­ tates development of a rapid methodology to characterize deviations from the wildtype se­ quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. 
  Reference    Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 
  Published    1990 
  Keywords    Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0418.pdf 
 Identifier    ZNC-1990-45c-0418 
 Volume    45 
2Author    Z. NaturforschRequires cookie*
 Title    Two Separate Genes Encode the Catalytic 70 kDa V-ATPase Subunit in Psilotum and Equisetum  
 Abstract    T hom as Starke, T im othy P. L inkkila, an d Jo h a n n Peter G o g arten Department o f Molecular and Cell Biology, U niversity o f Connecticut, Vacuolar type ATPases have been found on various endomem branes o f eukaryotic cells, e.g. lysosomes, chromaffin granules, vesicles derived from the G olgi apparatus, endosom es and vacuoles. Although this ATPase type is targeted to different compartments in one cell, only one gene for each subunit had been found per genome. Using PCR across intron-exon boundaries we show that two different genes encode the cat­ alytic subunit o f the V-ATPase in Psilotum nudum and Equisetum arvense. The substitution rates for the three codon positions and the intervening sequences show that in Psilotum both genes are transcribed and are under selection pressure, however, this seems not to be the case for Equisetum. The relatively high similarity between the two genes found in each species as compared to the interspecies similarities suggest that for som e time after the gene duplication had occurred the two copies were subject to gene conversion mechanisms. An unexpected de­ gree o f conservation o f the intervening sequences themselves is noted and statistically verified, however, no structural constraints that could explain these findings were detected. 
  Reference    Z. Naturforsch. 46c, 613 (1991); received April 5 1991 
  Published    1991 
  Keywords    V-ATPase, Intron-Exon Boundaries, Gene Duplication, Evolution, Polymerase Chain Reaction 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0613.pdf 
 Identifier    ZNC-1991-46c-0613 
 Volume    46 
3Author    RikK. Ibak, Lincoln TaizRequires cookie*
 Abstract    The classification of methanogenic bacteria as archaebacteria based on 16 s rR N A sequence analysis is currently in dispute. To provide an alternative m olecular marker, the polymerase chain reaction technique was used to amplify a 930 bp fragment o f Methanococcus thermolithotrophicus 
  Reference    Z. Naturforsch. 44c, 641 (1989); received April 6 1989 
  Published    1989 
  Keywords    M olecular Evolution, Methanobacteria, A rchaebacteria, A T P ase, Polymerase Chain Reaction 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0641.pdf 
 Identifier    ZNC-1989-44c-0641 
 Volume    44