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'Polymerase Chain Reaction' in keywords Facet   Publication Year 1991  [X]
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1991[X]
1Author    Z. NaturforschRequires cookie*
 Title    Two Separate Genes Encode the Catalytic 70 kDa V-ATPase Subunit in Psilotum and Equisetum  
 Abstract    T hom as Starke, T im othy P. L inkkila, an d Jo h a n n Peter G o g arten Department o f Molecular and Cell Biology, U niversity o f Connecticut, Vacuolar type ATPases have been found on various endomem branes o f eukaryotic cells, e.g. lysosomes, chromaffin granules, vesicles derived from the G olgi apparatus, endosom es and vacuoles. Although this ATPase type is targeted to different compartments in one cell, only one gene for each subunit had been found per genome. Using PCR across intron-exon boundaries we show that two different genes encode the cat­ alytic subunit o f the V-ATPase in Psilotum nudum and Equisetum arvense. The substitution rates for the three codon positions and the intervening sequences show that in Psilotum both genes are transcribed and are under selection pressure, however, this seems not to be the case for Equisetum. The relatively high similarity between the two genes found in each species as compared to the interspecies similarities suggest that for som e time after the gene duplication had occurred the two copies were subject to gene conversion mechanisms. An unexpected de­ gree o f conservation o f the intervening sequences themselves is noted and statistically verified, however, no structural constraints that could explain these findings were detected. 
  Reference    Z. Naturforsch. 46c, 613 (1991); received April 5 1991 
  Published    1991 
  Keywords    V-ATPase, Intron-Exon Boundaries, Gene Duplication, Evolution, Polymerase Chain Reaction 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0613.pdf 
 Identifier    ZNC-1991-46c-0613 
 Volume    46