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'Polyethylene Glycol' in keywords
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1981 (1)
1980 (1)
1978 (1)
1Author    AlanC. Cassells, FrancesM. CockerRequires cookie*
 Title    TMV Inoculation of Tobacco Protoplasts in the Presence of Protoplast Fusion Agents  
 Abstract    Two methods used to induce protoplast fusion, involving polyethylene glycol (PEG) and high calcium/high pH, respectively, have been investigated in procedures for the inoculation of isolated tobacco protoplasts with TMV. It has been shown that inoculation in the presence of fusion agents involves two stages. Firstly, virus precipitation on to the protoplast surface and secondly, entrapment of virus particles between protoplasts in zones (putatively) o f transient protoplast fusion. 
  Reference    Z. Naturforsch. 35c, 1057—1061 (1980); received June 24/August 22 1980 
  Published    1980 
  Keywords    Polyethylene Glycol, Protoplasts, Tobacco Mosaic Virus 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1057.pdf 
 Identifier    ZNC-1980-35c-1057 
 Volume    35 
2Author    H. Erm, Ann KrählingRequires cookie*
 Title    Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions  
 Abstract    Investigations on the dose response of cell fusion, induced by ionfree aqueous polyethylene glycol (PEG) solutions, reveal distinct lowest fusogenic PEG concentrations for different permanently growing mammalian cell lines. Part o f the requisite PEG can be replaced by carbo­ hydrates, preserving the fusogenity o f the solutions. This discriminates two effects o f PEG solutions causing cell fusion: a) cell shrinkage, the required hyperosmolality o f the solutions may be provided by PEG or by carbohydrates, is supposed to cause intracellular processes necessary for consolidating polycaryons; b) membrane alterations, which can not be induced by carbo­ hydrates, enable intimate cell-cell contact via particle-free membrane areas. Depending on cell line salts can not only raise the osmolality o f PEG solutions but are able to co-operate with PEG in generating membrane alterations. 
  Reference    Z. Naturforsch. 36c, 593—596 (1981); received March 111981 
  Published    1981 
  Keywords    Cell Fusion, Polyethylene Glycol, Fusogenic Properties, Carbohydrates, Salts 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0593.pdf 
 Identifier    ZNC-1981-36c-0593 
 Volume    36 
3Author    J.R 0 Dawson, P. E. Dickerson, JanetM. King, F. Sakai, A.R H Trim, J. W. WattsRequires cookie*
 Title    Improved Methods for Infection of Plant Protoplasts with Viral Ribonucleic Acid  
 Abstract    Infection of tobacco protoplasts with R N A of tobacco mosaic virus and the bromoviruses has been studied. The quality of the R N A was very important. Ribonuclease activity was controlled by incorporating 5 mM ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus R N A but infection with T M V R N A was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral R N A examined. 
  Reference    Z. Naturforsch. 33c, 548—551 (1978); received May 10 1978 
  Published    1978 
  Keywords    Protoplasts, R N A, Plant Viruses, Tobacco M osaic Virus, Polyethylene Glycol 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0548.pdf 
 Identifier    ZNC-1978-33c-0548 
 Volume    33