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'Poly L arginine' in keywords Facet   section ZfN Section C:Volume 035  [X]
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1980 (1)
1Author    Alexandru Popescu, Stefan Antohi, Stefan Trasculescu, Adriana Aurescu, NicolaeM. Anolescu, Victor CiocnituRequires cookie*
 Title    Changes Induced by Pre-Fixation in Polycation Bacteriolysis and Surface Alterations Matching the Model of Wall Picnosis in Cell Lysis  
 Abstract    Pretreatments of B. subtilis and S. aureus cells with lower concentrations of fixative agents, led to modifications in bacteriolytic effect exerted by polyarginine and protamine: Glutaraldehyde blocked polycation bacteriolysis while formaldehyde and osmium tetroxide (0 s0 4) having no influence on polyarginine action, increased constantly the cell sensitivity to protamine in lower doses otherwise nonlytic; the sensitizing action also resulted in the extension of protamine bacteriolytic pattern including several staphylococcal strains; higher bacteriolytic doses of protamine were contrastively unable to lyse OsO? prefixed cells and gave an inconstant lytic value with formaldehyde treated bacteria. With higher concentrations, 0 s 0 4 preserved intactly its sensitizing action while formaldehyde displayed a decrease in its ability to sensitize B. subtilis cells to the lytic effect of protamine. Scaning electron microscopy of polycation treated cells showed prelytic lesions as surface granulations, shape and size modifications and cell splits. The interpretation of the results in terms of intra-and intermolecular adducts accompanied by con­ formational changes in surface macromolecules is discussed. It is concluded that the results match the model of polycation bacteriolysis by wall multizonal picnosis leading to surface splits and thereby triggering cell-lysis. 
  Reference    Z. Naturforsch. 35c, 805—810 (1980); received July 9 1980 
  Published    1980 
  Keywords    Poly-L-arginine, Protamine, Bacillus subtilis, Staphylococcus aureus, Glutaraldehyde 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0805.pdf 
 Identifier    ZNC-1980-35c-0805 
 Volume    35