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1989 (1)
1Author    H. Reinecke, L. Dunemann, G. SchwedtRequires cookie*
 Title    Polarographische Untersuchungen zur Bestimmung der Stabilität von Metall-Protein-Bindungen Polarographie Investigations for the Determination of the Stability of Metal-Protein Bonds  
 Abstract    Bonds betw een metals (especially copper) and a protein fraction (18,100 g/m ol) of a soya bean flour extract have been investigated. The binding capacity (304 nmol Cu/mg protein) and the binding stability (K = 1,046 • 103 in an amm onia buffer system) were determined by polarographic investigations. Changes in the polarogram caused by spiking the protein with metal ions were compared with effects in m odel substances. C ysteine, ethylenediam ine, oxalic acid and deriva­ tives o f benzoic acid were used as chelating agents. The effects o f functional groups on the metal-protein bonds were estim ated by the determ ination o f their different binding stabilities. 
  Reference    Z. Naturforsch. 44b, 767 (1989); eingegangen am 3. März 1989 
  Published    1989 
  Keywords    Speciation, Polarography Proteins, Copper, Binding Stability 
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 TEI-XML for    default:Reihe_B/44/ZNB-1989-44b-0767.pdf 
 Identifier    ZNB-1989-44b-0767 
 Volume    44