| | 1 | Author
| Wolfgang Knogge, Gottfried Weissenböck, Dieter Strack | Requires cookie* | | | Title
| Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity  | | | | Abstract
| This report describes the separation of components from a 4-coumarate: CoA ligase assay by means of liquid chromatography. With the aid o f polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure /»-coumaroyl-CoA using as a solvent 0.01% N H 4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on octadecylsilane-bonded silica stationary phase allows a discontinuous determination o f ligase activity. All components — ATP, Coenzyme A, p-coumaric acid, and the products AMP and p-coumaroyl-CoA — can be separated and accurately quantified within 20 min using a water-acetonitrile gradient, containing 1% phosphoric acid. The presented HPLC method may be used to affirm the accuracy of optical tests. | | | |
Reference
| Z. Naturforsch. 36c, 197—199 (1981); received December 91980 | | | |
Published
| 1981 | | | |
Keywords
| Avena sativa, Poaceae, Ligase, /?-Coumaroyl-CoA, Polyamide Column Chromatography, High Performance Liquid Chromatography | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0197.pdf | | | | Identifier
| ZNC-1981-36c-0197 | | | | Volume
| 36 | |
| 2 | Author
| H. D. Zinsmeister, N. Erb, A. Nahrstedt, R. Hegnauer, L. Tjon, Sie Fat | Requires cookie* | | | Title
| Das cyanogene Glykosid von Holcus mollis The Cyanogenic Glycoside of Holcus mollis | | | |
Reference
| Z. Naturforsch. 36c, 336 (1981); received December 191980 | | | |
Published
| 1981 | | | |
Keywords
| Holcus, Poaceae, Liliopsida, 2-/?-D-Glucopyranosyloxy-2S-butyronitrile, (S)-Heterodendrin | | | |
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| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-0336_n.pdf | | | | Identifier
| ZNC-1981-36c-0336_n | | | | Volume
| 36 | |
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