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1981 (2)
1Author    Wolfgang Knogge, Gottfried Weissenböck, Dieter StrackRequires cookie*
 Title    Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity  
 Abstract    This report describes the separation of components from a 4-coumarate: CoA ligase assay by means of liquid chromatography. With the aid o f polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure /»-coumaroyl-CoA using as a solvent 0.01% N H 4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on octadecylsilane-bonded silica stationary phase allows a discontinuous determination o f ligase activity. All components — ATP, Coenzyme A, p-coumaric acid, and the products AMP and p-coumaroyl-CoA — can be separated and accurately quantified within 20 min using a water-acetonitrile gradient, containing 1% phosphoric acid. The presented HPLC method may be used to affirm the accuracy of optical tests. 
  Reference    Z. Naturforsch. 36c, 197—199 (1981); received December 91980 
  Published    1981 
  Keywords    Avena sativa, Poaceae, Ligase, /?-Coumaroyl-CoA, Polyamide Column Chromatography, High Performance Liquid Chromatography 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0197.pdf 
 Identifier    ZNC-1981-36c-0197 
 Volume    36 
2Author    H. D. Zinsmeister, N. Erb, A. Nahrstedt, R. Hegnauer, L. Tjon, Sie FatRequires cookie*
 Title    Das cyanogene Glykosid von Holcus mollis The Cyanogenic Glycoside of Holcus mollis  
  Reference    Z. Naturforsch. 36c, 336 (1981); received December 191980 
  Published    1981 
  Keywords    Holcus, Poaceae, Liliopsida, 2-/?-D-Glucopyranosyloxy-2S-butyronitrile, (S)-Heterodendrin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0336_n.pdf 
 Identifier    ZNC-1981-36c-0336_n 
 Volume    36