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1982 (1)
1Author    Arie Rosner, Marian Gorecki, Haim AvivRequires cookie*
 Title    Screening for Highly Active Plasmid Promoters via Fusion to /?-Galactosidase Gene  
 Abstract    A plasmid containing promoter-deleted inactive /?-galactosidase gene [1] was used to select promoters of the pEP121 plasmid [2]. Colonies o f cells harboring reactivated /?-galactosidase gene were identified by their red color on McConkey plates. The quantitative amounts o f /?-galactosidase produced in each clone were estimated by assaying enzyme activity and by measuring the specific /?-galactosidase protein following fractionation o f total cells' proteins on polyacrylamide gel. A wide range o f enzyme activities was observed. The most active promoter isolated was shown to promote /?-galactosidase production more efficiently, compared with the original /?-galactosidase promoter, amounting to 20% o f all cell proteins. Such highly active promoters may be utilized in the future, to promote expression o f cloned genes in bacteria. 
  Reference    Z. Naturforsch. 37c, 441—444 (1982); received January 13 1982 
  Published    1982 
  Keywords    Plasmid Promoter, /?-Galactosidase Gene, Escherichia coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0441.pdf 
 Identifier    ZNC-1982-37c-0441 
 Volume    37