| | 1 | Author
| Arie Rosner, Marian Gorecki, Haim Aviv | Requires cookie* | | | Title
| Screening for Highly Active Plasmid Promoters via Fusion to /?-Galactosidase Gene  | | | | Abstract
| A plasmid containing promoter-deleted inactive /?-galactosidase gene [1] was used to select promoters of the pEP121 plasmid [2]. Colonies o f cells harboring reactivated /?-galactosidase gene were identified by their red color on McConkey plates. The quantitative amounts o f /?-galactosidase produced in each clone were estimated by assaying enzyme activity and by measuring the specific /?-galactosidase protein following fractionation o f total cells' proteins on polyacrylamide gel. A wide range o f enzyme activities was observed. The most active promoter isolated was shown to promote /?-galactosidase production more efficiently, compared with the original /?-galactosidase promoter, amounting to 20% o f all cell proteins. Such highly active promoters may be utilized in the future, to promote expression o f cloned genes in bacteria. | | | |
Reference
| Z. Naturforsch. 37c, 441—444 (1982); received January 13 1982 | | | |
Published
| 1982 | | | |
Keywords
| Plasmid Promoter, /?-Galactosidase Gene, Escherichia coli | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/37/ZNC-1982-37c-0441.pdf | | | | Identifier
| ZNC-1982-37c-0441 | | | | Volume
| 37 | |
|
