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1991 (1)
1Author    M. Baier, W. HartungRequires cookie*
 Title    Cytoplasmic pH and Abscisic Acid Transport across Guard Cell Membranes  
 Abstract    The cytoplasmic pH value o f guard cells o f Valerianella locusta was determined from the distribution o f the weak acid [l4C]ABA between the medium and the cell, which was shown to be 8.1 ± 0.1. Treatment o f guard cells in media with low pH (pH 5.0) and different concentra­ tions o f sodium acetate (0 -5 m o lm '3) caused a considerable acidification o f the cytoplasm (6 .6 -7 .7). Using the compartmental analysis we determined also the fluxes o f ABA at the plas­ ma membrane o f guard cells (<I>co) and at the tonoplast o f guard cells (<t>cv) under steady state conditions. The flux o f ABA from the cytoplasm into the vacuoles (tonoplast flux) was de­ pendent upon the cytoplasmic pH. The data give evidence that A B A " is transported across the tonoplast by a saturable transport com ponent. The A BA flux at the plasma membrane seemed to be independent o f the cytoplasmic pH. 
  Reference    Z. Naturforsch. 46c, 106—110 (1991); received September 24 1990 
  Published    1991 
  Keywords    ABA Transport, Cytoplasmic pH, Guard Cells, Plasmamembrane, Tonoplast 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0106.pdf 
 Identifier    ZNC-1991-46c-0106 
 Volume    46