| | 1 | Author
| JochenB. Erlin, +., Peter Kiss, D. Ieter, M. Üller-Enoch, D. Ieter Gierse, W. Olfgang Barz, B. Oris Janistyn | Requires cookie* | | | Title
| Über den Abbau von Chalkonen und Isoflavonen in pflanzlichen Zellsuspensionskulturen Degradation of Chalcones and Isoflavones in P lant Cell Suspension Cultures  | | | | Abstract
| Cell suspension culture of Phaseolus aureus, Glycine max and Pisum sativum have been used to determine the extent of chalcone and isoflavone catabolism. The A-rings of calcones and isoflavones with both resorcinol and phloroglucinol pattern of sub stitution have unequivocally been shown to be degraded as measured by carbon dioxide production The earlier described catabolic pathway of chalcones with the B-ring liberated as a substituted benzoic acid has been verified using another chalcone. With the use of various u C-labelled isoflavones it could be demonstrated that essentially all carbon atoms are introduced into catabolic reactions. Incorporation of 4',7-dihydroxyisoflavone (daidzein) into insoluble polymeric material has been shown to proceed via the 3',4',7-trihydroxyisoflavone. 3'-Hydroxylation and subsequent polymeri sation of the orf/io-dihydroxy compound can completely be inhibited by using anaerobic conditions which favour glucoside formation instead. 4'-0-Methylgroups in isoflavones prevent the phenolase catalzyed 3'-hydroxylation and thus the incorporation of isoflavones into polymeric structures. 6,7-Dihydroxy substituted insoflavones when fed to cell cultures are not polymerized by phenolase but are rather converted to glycosides. | | | |
Reference
| (Z. Naturforsch. 29c, 374 [1974]; eingegangen am 1. April 1974) | | | |
Published
| 1974 | | | |
Keywords
| Chalcones, Isoflavones, Catabolism, Plant Cell Cultures | | | |
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| | | | | TEI-XML for
| default:Reihe_C/29/ZNC-1974-29c-0374.pdf | | | | Identifier
| ZNC-1974-29c-0374 | | | | Volume
| 29 | |
| 2 | Author
| Hans-Ullrich Gebauer, Ursula Seitz, Ulrich Seitz | Requires cookie* | | | Title
| Transport and Processing of Ribosomal R N A in Plant Cells after Treatment with Cycloheximide | | | | Abstract
| In freely suspended cells of parsley (P etroselin u m crispu m) protein synthesis is inhibited nearly totally by 5 /ug/m l cycloheximide within 10 min. This very low dose has a slight effect on the rRNA synthesis too. During an incubation period of 60 min with [32P] orthophosphate in the presence of the inhibitor (5 /ugjml) at least 65 — 70% mature rRNA are synthesized compared with the control. After 120 min the synthesis is progressively reduced to 60 — 65%, and after 240 min to 30-40% . Cycloheximide causes a delay of the processing of the precursor RNA species leading to an accumulation of these components. In addition to the 2.3 x 10® daltons RNA, normally detectable in pulse experiments, two further precursor molecules do emerge which under normal circumstances apparently are shortlived. Their molecular weights are 2.0 x 10® and 0.9 x 10® daltons. The pulse-chase technique and cell fractionation into nuclear and ribosomal parts enables us to demonstrate a rapid transfer of labelled 18S and 25S RNA during a 15 min chase treatment to the mature cytoplasmic ribosomes. Under these conditions no differential transport of the two com ponents takes place. The cells possess a pool of proteins. Therefore the formation of RNP-particles or ribosomes respectively and the transport from the nucleus to the cytoplasm are independent from protein synthesis for some time. | | | |
Reference
| (Z. Naturforsch. 30c, 213 [1975]; received November 25 1974) | | | |
Published
| 1975 | | | |
Keywords
| Ribosomal RNA, Nuclei, Ribosomes, Cycloheximide, Plant Cell Culture | | | |
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| default:Reihe_C/30/ZNC-1975-30c-0213.pdf | | | | Identifier
| ZNC-1975-30c-0213 | | | | Volume
| 30 | |
| 5 | Author
| Barbara Upmeier, JürgenE. Thomzik, Wolfgang Barz | Requires cookie* | | | Title
| Enzymatic Studies on the Reversible Synthesis of Nicotinic Acid-N-glucoside in Heterotrophic Parsley Cell Suspension Cultures | | | | Abstract
| A soluble enzyme catalyzing the transfer of the glucose moiety from UDP-glucose to the nitrogen atom of nicotinic acid was detected in protein preparations from heterotrophic cell suspension cultures of parsley (Petroselinum hortense Hoffm.). Enzyme activity was enriched 22-fold by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The UDP-glucose : nicotinic acid-N-glucosyltransferase showed a pH-optimum at pH 7.8—8.2 and a temperature optimum at 30 °C. The apparent K M values were determined to be 170 ^M for nicotinic acid and 1.2 mM for the cosubstrate. The native enzyme had a molecular mass of about 46 kDa. The glucosyltransferase reaction was shown to be reversible. The transfer of the glucose molecule from nicotinic acid-N-glucoside to uridinediphosphate yielding uridinediphosphoglucose and nicotinic acid could be demonstrated indicating that nicotinic acid-N-glucoside has a high group-transfer potential. | | | |
Reference
| Z. Naturforsch. 43c, 835—842 (1988); received August 1/September 30 1988 | | | |
Published
| 1988 | | | |
Keywords
| Petroselinum hortense, Umbelliferae, Plant Cell Cultures, Nicotinic Acid, Nicotinic Acid-N-glucoside | | | |
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| default:Reihe_C/43/ZNC-1988-43c-0835.pdf | | | | Identifier
| ZNC-1988-43c-0835 | | | | Volume
| 43 | |
| 6 | Author
| Z. Naturforsch | Requires cookie* | | | Title
| Nicotinic Acid Conjugation in Plants and Plant Cell Cultures of Potato (Solanum tuberosum) | | | | Abstract
| S usanne K öster, B a rb a ra U p m eier, D ie te r K om ossa, an d W olfgang B arz Lehrstuhl für Biochem ie der Pflanzen, W estfälische W ilhelm s-U niversität M ünster, Hindenburg-platz The conversion o f nicotinic acid to N-conjugates by either m ethylation or glucosylation has been investigated in leaves and tubers from potato plants, in potato callus and suspension cultures and in plants regenerated from such cultures. While nicotinic acid-N-glucosyltransferase activity could be detected in the cells and tissues at each level of differentiation, the N-methyltransferase (trigonelline formation) was only found in organized leaf tissue. | | | |
Reference
| Z. Naturforsch. 44c, 623 (1989); received February 24/M arch 28. 1989 | | | |
Published
| 1989 | | | |
Keywords
| Solanum tuberosum, Solanaceae, Plant Cell Cultures, N icotinic A cid, Trigonelline | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0623.pdf | | | | Identifier
| ZNC-1989-44c-0623 | | | | Volume
| 44 | |
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