| | 1 | Author
| Christine Schindler, Ruth Hracky, Jürgen Soll | Requires cookie* | | | Title
| Protein Transport in Chloroplasts: ATP is Prerequisit  | | | | Abstract
| The energy requirem ent for protein transport into chloroplast was assayed under conditions that perm it to distinguish whether the posttranslational translocation is dependent on A TP or w hether a m em brane potential across the chloroplast envelope can drive this transport event. A m em brane potential is not required for translocation. A TP can support protein transport in the presence of protonophores and ionophores. Non-hydrolyzable A TP analogues and G TP, CTP, U T P cannot serve as ATP substitutes. Translocation could be observed when an A TP generating system was used to supply ATP. In contrast ATP degrading systems completely abolished translocation. The inner envelope mem brane localized ATP-ase is probably not involved in the transport event. The results suggest that ATP is needed at the outer chloroplast envelope. Inhibition of protein transport by A D P , pyrophosphate and NaF is studied and its con sequences discussed. | | | |
Reference
| Z. Naturforsch. 42c, 103 (1987); received August 26 1986 | | | |
Published
| 1987 | | | |
Keywords
| Protein T ransport, ATP-Hydrolysis, Chloroplasts, Envelope, Pisum sativum | | | |
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| default:Reihe_C/42/ZNC-1987-42c-0103.pdf | | | | Identifier
| ZNC-1987-42c-0103 | | | | Volume
| 42 | |
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