| 1 | Author
| P. Hefferle, P. Geiselhart, T. Mindl, S. Schneider, W. John, H. Scheer | Requires cookie* | | Title
| Time-Resolved Polarized Fluorescence of C-Phycocyanin and Its Subunits from Mastigocladus laminosus  | | | Abstract
| The influence o f aggregation and temperature on the excited state kinetics o f C -phycocyanin from Mastiqocladus laminosus has been studied. Polarized fluorescence decay curves have been recorded using a synchronously pum ped dye laser in conjunction with a synchroscan streak camera. The experimental data for all sam ples can be fit satisfactorily assum ing a biexponential decay law. Fluorescence depolarization tim es have been interpreted in terms o f energy transfer among the different chromophores. The influence o f temperature is only m oderate on the intra molecular relaxation, but pronounced on the rates o f energy transfer. Both are dependent on the size o f the aggregate. The biexponential decay o f the a-subunit containing only one Chromo phore, indicates the presence o f different subsets o f chrom ophores in these sam ples. The results are discussed in terms o f variations o f the chrom ophore arrangem ents upon temperature induced changes in the protein conformation. | | |
Reference
| Z. Naturforsch. 39c, 606 (1984); received February 16 1984 | | |
Published
| 1984 | | |
Keywords
| Photosynthesis, Biliproteins, Picosecond Spectroscopy, Energy Transfer, Protein D enaturation | | |
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| default:Reihe_C/39/ZNC-1984-39c-0606.pdf | | | Identifier
| ZNC-1984-39c-0606 | | | Volume
| 39 | |
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