| | 1 | Author
| Peter Westhoff, Kurt Zimmermann, Frank Boege, Klaus Zetsche | Requires cookie* | | | Title
| Regulation of the Synthesis of Ribulose-l,5-bisphosphate Carboxylase and Its Subunits in the Flagellate Chlorogonium elongatum. II. Coordinated Synthesis of the Large and Small Subunits  | | | | Abstract
| Transfer of heterotrophically grown cells of the unicellular green alga Chlorogonium elongatum to autotrophic growth conditions causes a 10 -1 5 fold increase in the amount o f the chloroplastic enzyme ribulose-1,5-bisphosphate carboxylase. This increase was found to be due to de novo synthesis. The relative proportions o f large and small subunits o f the enzyme do not change. Their ratio is close to 3.4, the proportions in weight o f the two subunits in the holoenzyme. Continous labelling with [35S]sulfate reveals that the ratios of incorporation into large and small subunits are essentially the same in autotrophic and heterotrophic cells. Pulse-chase experiments show that the subunits are degraded synchronously. The coordinated subunit synthesis cannot be uncoupled using inhibitors o f protein and RNA synthesis or high temperature of cultivation of the alga. The results suggests a very tightly coordinated synthesis o f the large and small subunits of ribulosebisphosphate carboxylase. | | | |
Reference
| Z. Naturforsch. 36c, 942 (1981); received July 18 1981 | | | |
Published
| 1981 | | | |
Keywords
| Ribulose-1, 5-bisphosphate Carboxylase, Synthesis, Subunits, Regulation, Phytoflagellate Chlorogonium elongatum | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-0942.pdf | | | | Identifier
| ZNC-1981-36c-0942 | | | | Volume
| 36 | |
|
