| | 1 | Author
| T. Hom As Brandlm, Hugo Scheer, W. Olfhart Rüdiger | Requires cookie* | | | Title
| Chromophore Content and Molar Absorptivity of Phytochrome in the Pr Form  | | | | Abstract
| Quantitative absorption spectra in the visible and UV region were recorded for denaturated phytochrome (P r) and phytochromobilin peptides in comparison with native Pr. The method was tested with C-phycocyanin from Spirulina platensis. Based on known molar absorptivities for denaturated phycocyanin and suitable model compounds, and on the ratio ^niüve/^delrS = 2.9, ^native was determined to be 1 0 2 0 0 0 m _1 cm-1 for one phycocyanobilin chromophore in native phycocyanin. Likewise, N a t iv e was calculated to be 19000 M -1 cm-1. The corresponding ratios for Pr Were ^native/^denatured = 3*4 and A native/-^degraded = 3.7, t h l S yielded ^native = 109000 tO 118000 and eSUfcve = 36000 M _1 cm-1 for phytochromobilin in native P r. This value corresponds to one phytochromobilin per small phytochrome (60000 D) if data from the literature are corrected for the content o f colorless proteins. The latter has been asessed from (i) the purity index (A2g0/A665) and (ii) the contribution o f the phytochromobilin chromophore at 280 nm as derived from model compounds. | | | |
Reference
| Z. Naturforsch. 36c, 431 (1981); received February 20 1981 | | | |
Published
| 1981 | | | |
Keywords
| Absorption, Spectroscopy, A-Dihydrobilindiones, Isophorcabilin, Phycobiliproteins, Phytochrome, Phytochromobilin-Peptides | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0431.pdf | | | | Identifier
| ZNC-1981-36c-0431 | | | | Volume
| 36 | |
| 2 | Author
| Fritz Thüm, Thom Ler, W. As Brandlmeier, Olfhart Rüdiger | Requires cookie* | | | Title
| Preparation and Properties of Chromopeptides from the Pfr Form of Phytochrome | | | | Abstract
| Chromopeptides were prepared from the Pfr form o f phytochrome by pepsin digestion. After separation from colorless peptides and Pr peptides by column chromatography, spectral characteristics of this Pfr peptide were determined (A^ax = 610nm, A£ax = 380nm in acid methanol). Irradiation of Pfr peptide produces Pr peptide without liberation of a detectable compound. The Pfr peptide is more sensitive to oxidation and reduction than the Pr peptide. Oxidation with iodine and reduction with dithionite leads to partial chemoconversion o f the Pfr peptide to the Pr peptide. The results favor the model o f cis-trans isomerization for the Pr ^ Pfr transformation. | | | |
Reference
| Z. Naturforsch. 36c, 440 (1981); received February 201981 | | | |
Published
| 1981 | | | |
Keywords
| Avena sativa L, Phytochrome, Pepsin Digestion, Phytochromobilin Peptide, Pfr Peptide | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0440.pdf | | | | Identifier
| ZNC-1981-36c-0440 | | | | Volume
| 36 | |
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