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'Phytochrome' in keywords Facet   Publication Year 1981  [X]
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1Author    T. Hom As Brandlm, Hugo Scheer, W. Olfhart RüdigerRequires cookie*
 Title    Chromophore Content and Molar Absorptivity of Phytochrome in the Pr Form  
 Abstract    Quantitative absorption spectra in the visible and UV region were recorded for denaturated phytochrome (P r) and phytochromobilin peptides in comparison with native Pr. The method was tested with C-phycocyanin from Spirulina platensis. Based on known molar absorptivities for denaturated phycocyanin and suitable model compounds, and on the ratio ^niüve/^delrS = 2.9, ^native was determined to be 1 0 2 0 0 0 m _1 cm-1 for one phycocyanobilin chromophore in native phycocyanin. Likewise, N a t iv e was calculated to be 19000 M -1 cm-1. The corresponding ratios for Pr Were ^native/^denatured = 3*4 and A native/-^degraded = 3.7, t h l S yielded ^native = 109000 tO 118000 and eSUfcve = 36000 M _1 cm-1 for phytochromobilin in native P r. This value corresponds to one phytochromobilin per small phytochrome (60000 D) if data from the literature are corrected for the content o f colorless proteins. The latter has been asessed from (i) the purity index (A2g0/A665) and (ii) the contribution o f the phytochromobilin chromophore at 280 nm as derived from model compounds. 
  Reference    Z. Naturforsch. 36c, 431 (1981); received February 20 1981 
  Published    1981 
  Keywords    Absorption, Spectroscopy, A-Dihydrobilindiones, Isophorcabilin, Phycobiliproteins, Phytochrome, Phytochromobilin-Peptides 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0431.pdf 
 Identifier    ZNC-1981-36c-0431 
 Volume    36 
2Author    Fritz Thüm, Thom Ler, W. As Brandlmeier, Olfhart RüdigerRequires cookie*
 Title    Preparation and Properties of Chromopeptides from the Pfr Form of Phytochrome  
 Abstract    Chromopeptides were prepared from the Pfr form o f phytochrome by pepsin digestion. After separation from colorless peptides and Pr peptides by column chromatography, spectral characteristics of this Pfr peptide were determined (A^ax = 610nm, A£ax = 380nm in acid methanol). Irradiation of Pfr peptide produces Pr peptide without liberation of a detectable compound. The Pfr peptide is more sensitive to oxidation and reduction than the Pr peptide. Oxidation with iodine and reduction with dithionite leads to partial chemoconversion o f the Pfr peptide to the Pr peptide. The results favor the model o f cis-trans isomerization for the Pr ^ Pfr transformation. 
  Reference    Z. Naturforsch. 36c, 440 (1981); received February 201981 
  Published    1981 
  Keywords    Avena sativa L, Phytochrome, Pepsin Digestion, Phytochromobilin Peptide, Pfr Peptide 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0440.pdf 
 Identifier    ZNC-1981-36c-0440 
 Volume    36