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1984 (1)
1979 (1)
1Author    Erhard Sandm, Ann, Willy HilgenbergRequires cookie*
 Title    Der Gehalt an Gallus-und Protocatechusäure in Abhängigkeit vom Entwicklungszustand von Phycomyces blakesleeanus Bgff. G allic and Protocatechuic Acid Content during the Development of Phycom yces bla­ kesleeanus  
 Abstract    A quantitative determ ination method o f gallic and protocatechuic acid in cultures and liquid nutrient o f Phycom yces blakesleeanus was described. Both phenolic acids were separated by TLC and the colour reaction with Folin reagent was used for a colorimetric test. This procedure was em ployed for investigating the formation o f gallic and protocatechuic acid in cultures with opti­ mal (10-4 m) and reduced (1.3 x 10-6 m) zinc supply showing that their production is stim ulated by zinc ions. In addition, the inhibiting effect o f light on the accum ulation o f gallic acid was m anifested, how ­ ever, its excretion into the m edium was uneffected by light and protocatechuic acid was not ex­ creted at all. During the developm ent o f Phycomyces gallic and protocatechuic acid could be detected in two days old m ycelium . With the sporangiophore production both acids are accum ulated more rapid­ ly in the sporangiophores. After the end o f sporangiophore formation the gallic acid content in­ creases only slightly. In contrast the total content o f protocatechuic acid decreases sharply. As no excretion occurs a degradation o f at least protocatechuic acid must be taken into consideration. 
  Reference    Z. Naturforsch. 34c, 747—750 (1979); eingegangen am 2. Juli 1979 
  Published    1979 
  Keywords    Phycomyces blakesleeanus, G allic Acid, Protocatechuic Acid, Zinc Stim ulation, Light Inhibition 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0747.pdf 
 Identifier    ZNC-1979-34c-0747 
 Volume    34 
2Author    PeterM. Bramley, IanE. Clarke, K., Gerhard Sandmann, Peter BögerRequires cookie*
 Title    Inhibition of Carotene Biosynthesis in Cell Extracts of Phycomyces blakesleeanus  
 Abstract    Cell extracts o f the C l 15 (^-carotene-accum ulating) strain o f Phycom yces blakesleeanus were incubated with either [2-l4C]MVA or [1-I4C]IPP and a range o f possible inhibitors o f caro-tenogenesis, including bleaching herbicides, biphenyl com pounds and geranylacetone. Several o f these compounds were potent inhibitors o f /7-carotene form ation and caused the accum ulation o f phytoene. N o other carotenes were found to accum ulate, in vitro. The structures o f these inhibi­ tors, compared to that o f phytoene, suggest that they affect the enzym ic activity o f "phytoene dehydrogenase", possibly by com petitive inhibition. 
  Reference    Z. Naturforsch. 39c, 460—463 (1984); received N ovem ber 1 1983 
  Published    1984 
  Keywords    Carotenoid Biosynthesis, Bleaching Herbicides, Inhibitors o f Phytoene Desaturation, Phycomyces blakesleeanus, Cell Extracts 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0460.pdf 
 Identifier    ZNC-1984-39c-0460 
 Volume    39