| | 1 | Author
| W. Kufer, O. Schmid, G. Schmidt, H. Scheer | Requires cookie* | | | Title
| The Diazo Reaction of Bilirubins and Phycorubins: A Quantitative Study  | | | | Abstract
| A quantitative study o f the diazo reaction has been made with several bilirubins including a 2,3-dihydrobilirubin and several phycorubins. The latter have been prepared from the integral phy-cocyanin o f two cyanobacteria (M astigocladus laminosus and Spirulina platen sis), and from the phycocyanin subunits. T hese phycorubins have been subjected to the diazo reaction in order to test for the presence o f a second covalent chromophore protein bond in the latter. | | | |
Reference
| Z. Naturforsch. 41c, 437—452 (1986); received Novem ber 21 1985 | | | |
Published
| 1986 | | | |
Keywords
| Photosynthesis, Phycobiliproteins, A ntenna, Bile Pigm ents, Biliverdin | | | |
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| default:Reihe_C/41/ZNC-1986-41c-0437.pdf | | | | Identifier
| ZNC-1986-41c-0437 | | | | Volume
| 41 | |
| 2 | Author
| T. Hom As Brandlm, Hugo Scheer, W. Olfhart Rüdiger | Requires cookie* | | | Title
| Chromophore Content and Molar Absorptivity of Phytochrome in the Pr Form | | | | Abstract
| Quantitative absorption spectra in the visible and UV region were recorded for denaturated phytochrome (P r) and phytochromobilin peptides in comparison with native Pr. The method was tested with C-phycocyanin from Spirulina platensis. Based on known molar absorptivities for denaturated phycocyanin and suitable model compounds, and on the ratio ^niüve/^delrS = 2.9, ^native was determined to be 1 0 2 0 0 0 m _1 cm-1 for one phycocyanobilin chromophore in native phycocyanin. Likewise, N a t iv e was calculated to be 19000 M -1 cm-1. The corresponding ratios for Pr Were ^native/^denatured = 3*4 and A native/-^degraded = 3.7, t h l S yielded ^native = 109000 tO 118000 and eSUfcve = 36000 M _1 cm-1 for phytochromobilin in native P r. This value corresponds to one phytochromobilin per small phytochrome (60000 D) if data from the literature are corrected for the content o f colorless proteins. The latter has been asessed from (i) the purity index (A2g0/A665) and (ii) the contribution o f the phytochromobilin chromophore at 280 nm as derived from model compounds. | | | |
Reference
| Z. Naturforsch. 36c, 431 (1981); received February 20 1981 | | | |
Published
| 1981 | | | |
Keywords
| Absorption, Spectroscopy, A-Dihydrobilindiones, Isophorcabilin, Phycobiliproteins, Phytochrome, Phytochromobilin-Peptides | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0431.pdf | | | | Identifier
| ZNC-1981-36c-0431 | | | | Volume
| 36 | |
| 3 | Author
| S. Schneider, H. Tiltscher, R. Fischer, D. H. Scheer | Requires cookie* | | | Title
| UV-VIS Absorption Spectra at High Pressure of C-Phycocyanin and Allophycocyanin from Mastigocladus laminosus | | | | Abstract
| The behaviour o f monom ers and trimers o f C-phycocyanin and allophycocyanin under high pressure is studied by U V-V IS absorption spectroscopy. It is found that fast variations o f pres sure (Ap > 500 bar) and/or temperature are accompanied by significant changes in the absorp tion spectra (intensity and/or spectral shift). The induced differences disappear, however, in part, when the samples are left for several minutes at the final pressure (relaxation effect). The observed spectral variations are different from those connected with a change in state o f aggre gation and could therefore be due to small m odifications o f the chromophore-protein arrange ment. | | | |
Reference
| Z. Naturforsch. 46c, 717 (1991); received April 29 1991 | | | |
Published
| 1991 | | | |
Keywords
| gewidmet Photosynthesis, Phycobiliproteins, Light-Harvesting Pigments, Aggregation Effects, High-Pressure Spectroscopy | | | |
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| default:Reihe_C/46/ZNC-1991-46c-0717.pdf | | | | Identifier
| ZNC-1991-46c-0717 | | | | Volume
| 46 | |
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