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1996 (1)
1Author    Gerhard Sandmann3, Christian Schneiderb, Peter BögerbRequires cookie*
 Title    A New Non-Radioactive Assay of Phytoene Desaturase to Evaluate Bleaching Herbicides  
 Abstract    A non-radioactive cell-free assay was developed to quantitatively determine inhibition of plant-type phytoene desaturase by bleaching herbicides. A n active desaturase was prepared from an appropriately cloned E. coli transformant. Another E. coli transformant was used to produce the required phytoene. Phytofluene and t-carotene, the products of the desaturase reaction, were either determined by H PL C or optical absorption spectra. Enzyme kinetics and inhibition data for the bleaching tetrazole herbicide W L 1 10547 are presented as an example. 
  Reference    Z. Naturforsch. 51c, 534—538 (1996); received April 26/May 3 1996 
  Published    1996 
  Keywords    Phytoene, Phvtofluene, u-Carotene, Phvtoene Desaturase, in-vitro Assay, /50 Value, W L 110547 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0534.pdf 
 Identifier    ZNC-1996-51c-0534 
 Volume    51