| | 1 | Author
| AchimE. Gau, Gudrun Wälzlein, Susanne Gärtner, Matthias Kuhlmann, Susanne Specht, ElfriedeK. Pistorius | Requires cookie* | | | Title
| Immunological Identification of Polypeptides in Photosystem II Complexes from the Cyanobacterium Anacystis nidulans  | | | | Abstract
| Photosystem II complexes from the cyanobacterium Anacystis nidulans have been investigated by Western blots with antisera raised against four photosystem II peptides from plants and with an antiserum raised against the soluble L-amino acid oxidase protein from/1. nidulans to achieve an iden tification of the polypeptides — especially of the L-amino acid oxidase related protein — in isolated photosystem II complexes. Anacystis photosystem II complexes which were solubilized with lauryldimethylamine N-oxide and purified by sucrose cushion and sucrose gradient centrifugation, contained as major Coomassie brilliant blue stained polypeptides a 71 kDa band of unknown identity, a 62 kDa band, which partly contained D-l, a 55 and 49 kDa band which were immuno-reactive with an antiserum to the 47 kDa peptide of tobacco PS II complexes, and three distinct bands in the 30 kDa region. These latter bands could be identified as the extrinsic Mn stabilizing peptide (27—30 kDa), D-l (30—33 kDa) and a 36 kDa peptide (35 — 38 kDa) which crossreacted with the antiserum raised against the soluble L-amino acid oxidase protein of 50 kDa. These results suggest that the 36 kDa peptide present in purified photosystem II complexes from A. nidulans might be a processed form of the soluble 50 kDa L-amino acid oxidase protein. | | | |
Reference
| Z. Naturforsch. 44c, 971—975 (1989); received June 22 1989 | | | |
Published
| 1989 | | | |
Keywords
| Photosystem II, L-Amino Acid Oxidase, Antibody, Cyanobacteria, Anacystis nidulans | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0971.pdf | | | | Identifier
| ZNC-1989-44c-0971 | | | | Volume
| 44 | |
| 2 | Author
| G. Ajlani, I. Meyer, C. Astier, C. Vernotte | Requires cookie* | | | Title
| Inhibition of Photosystem II by Ioxynil in Wild Type and Resistant Mutant of Synechocystis 6714 | | | | Abstract
| A Synechocystis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Ioxynil was shown to inhibit both the donor and the acceptor sides of photosystem II, but at different concentrations. The mutation found in the psbA gene (encoding the D, protein) at codon 266 (asparagine to threonine) [G. Ajlani, I. Meyer, C. Vernotte, and C. Astier, FEBS Lett. 246, 207—210 (1989)] gives a ten-fold resistance of the acceptor side to ioxynil without any modification of the sensitivity of the donor side. Electron transfer between the primary and the secondary acceptor of photosystem II was identical in the mutant and the wild type. The mutant remains sensitive to atrazine and is even more sensitive to D C M U than the wild type. | | | |
Reference
| Z. Naturforsch. 44c, 979 (1989); received June 16 1989 | | | |
Published
| 1989 | | | |
Keywords
| Phenolic Herbicide, Ioxynil, Photosystem II, Cyanobacteria, D i Protein | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0979.pdf | | | | Identifier
| ZNC-1989-44c-0979 | | | | Volume
| 44 | |
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