| | 1 | Author
| Matthias Kuhn, Andreas Thiel, Peter Böger | Requires cookie* | | | Title
| The 9-kDa Phosphoprotein Involved in Photoinhibition  | | | | Abstract
| Photosystem-II particles exhibit strong photoinhibition. Short-term illumination of photosys-tem-II particles with high-intensity light (5000 piE/m 2 x s) leads to a typical change of the protein pattern on SDS-PAGE. Two proteins are mainly affected, namely the well-described 32-kDa herbicide-binding protein which probably is degraded [1] and, first published here, the 9-kDa phosphoprotein, whose function in the PS-II complex is still unknown. This protein is not de-graded, but seems to be linked to other polypeptides of the PS-II complex. During light treatment new bands of 23, 41, 50 and 54 kDa appear in the protein pattern of SDS-PAGE. A monospecific antiserum was produced against the 9-kDa phosphoprotein to investigate its fate. After light treatment the antibodies reacted with new proteins of higher molecular weights, most pronounced with a 23-kDa and a 41-kDa peptide. | | | |
Reference
| Z. Naturforsch. 43c, 413—417 (1988); received February 15 1988 | | | |
Published
| 1988 | | | |
Keywords
| 9-kDa Phosphoprotein, Photosystem-II Particles, Photoinhibition, Protein Phosphorylation, Antibody | | | |
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| | | | | TEI-XML for
| default:Reihe_C/43/ZNC-1988-43c-0413.pdf | | | | Identifier
| ZNC-1988-43c-0413 | | | | Volume
| 43 | |
| 2 | Author
| J. Bednarz, A. Radunz, G. H. Schmid | Requires cookie* | | | Title
| Lipid Composition of Photosystem I and II in the Tobacco Mutant Nicotiana tabacum NC 95 | | | | Abstract
| The lipids of photosystem II particles, of chloroplasts and leaves are compared in the variegated tobacco mutant NC 95. The mutant differs from other N. tabacum mutants by the phenomenon that it has variegated leaves with green and with yellow-green leaf patches. Chloroplasts from the green leaf areas exhibit photosystem II and photosystem I reactions and have a normal lamellar system with grana and intergrana regions. Chloroplasts from the yellow-green leaf areas, how-ever, yield only photosystem I reactions and have only single stranded isolated thylakoids. Hence, this mutant offers the unique possibility to compare without the use of detergents within the same plant the lipid composition of photosystem II particles with that in intact chloroplasts, exhibiting either photosystem II and I reactions or those exhibiting exclusively photosystem I reactions. The lipids of photosystem II particles are composed of 37% glycolipids, 4% phospholipids, 5% carotenoids and 54% chlorophyll. Lipids of chloroplasts with grana stacking are composed of 75% glycolipids, 7% phospholipids, 2% carotenoids and 16% chlorophyll. Chloroplasts with single isolated thylakoids have a lipid composition consisting of 83% glycolipids, 14% phos-pholipids and only 0.5% carotenoids and 2% chlorophyll. The chloroplast lipid mixture is charac-terized in comparison to the respective leaf lipid mixture by a 16—17% higher glycolipid portion and by a 13-70% lower phospholipid content. The main difference in the lipid composition of photosystem I and II consists in the observation that chloroplasts active in only photosystem I contain more than double the amount of glycolipids and the 4-fold amount of phospholipids in comparison to photosystem II active preparations. The amount of monogalactolipid is even 3 times higher in chloroplasts active only in photosystem I when compared to those in photosystem II particles. In photosystem II particles phosphatidyl-ethanolamine is completely lacking and phosphatidylglycerol and phosphatidylinositol occur only in traces. The fatty acids of the sulfolipid are by 45% more saturated in the photosystem II particles and the digalactolipids of the photosystem II particles are by 28% more saturated than in chloroplasts exhibiting photosystem I and II activity. | | | |
Reference
| Z. Naturforsch. 43c, 423—430 (1988); received March 3 1988 | | | |
Published
| 1988 | | | |
Keywords
| Lipids, Fatty Acids, Chloroplasts, Lipid Asymmetry, Photosystem II Particles | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/43/ZNC-1988-43c-0423.pdf | | | | Identifier
| ZNC-1988-43c-0423 | | | | Volume
| 43 | |
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