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1996 (1)
1984 (1)
1Author    N. Pucheu, G.F W IldnerRequires cookie*
 Title    Isolation of 3 2 -3 5 kDa Thylakoid Proteins from Chlamydomonas reinhardii  
 Abstract    The protein com position o f Chlamydomonas reinhardii thylakoids in the m olecular weight range o f 3 2 -3 5 kDa was studied. The thylakoids were labelled with 32P —Pj in vivo using Pr starved cell cultures, solubilized with SDS and separated by polyacrylam ide gradient gel electrophoresis. The following differentiation o f proteins could be accom plished: two proteins were phosphorylated, which were also well stained with C oom assie blue, and one major protein was only detected by the silver staining procedure. The m obility o f the latter protein is different in gels with urea, showing an apparently lower molecular weight. In order to investigate whether a functional photosystem II is obligatory for protein phos­ phorylation, the phosphorylation o f thylakoid proteins was studied with a photosystem II deficient mutant. The mutant, which had normal photosystem I activity, but lacked photo­ system II activity, could synthesize ATP light dependently; its m ain labelled protein bands had a molecular weight o f 3 2 -3 5 kDa; it contained the light harvesting protein chlorophyll com plex and an unknown protein at 22 kDa. The 32P incorporation in photosystem II deficient cells was comparable to cells with functional photosystem II units. 
  Reference    Z. Naturforsch. 39c, 434 (1984); received N ovem ber 30 1983 
  Published    1984 
  Keywords    Photosystem II Complex, Q B-Protein, Chlamydomonas reinhardii, Protein Phosphorylation, Photosystem II Deficiency 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0434.pdf 
 Identifier    ZNC-1984-39c-0434 
 Volume    39 
2Author    P. He, A. Radunz, K. P. Bader, G. H. SchmidRequires cookie*
 Title    Influence of C 0 2 and S 0 2 on Growth and Structure of Photosystem II of the Chinese Tung-Oil Tree Aleurites montana  
 Abstract    Three months old plants of the Chinese tung-oil tree Aleurites montana were cultivated for 4 months in air containing an increased amount o f 700 ppm C 0 2. During the exposure to 700 ppm C 0 2 the plants exhibited a considerably stronger growth (3 0 -4 0 %) in compari­ son to the control plants (grown in normal air). In these C02-plants during the entire analyz­ ing period the amount of soluble proteins, of soluble sugars and the chlorophyll content were lower than in control plants. The protein content, referred to leaf area, increased during this time in both plant types by approx. 50% but with a different time course. The increase is faster in C02-plants compared to control plants, and ends up with similar values in both plants after 4 months. No difference is seen between sun and shade leaves. The chlorophyll content in both sun and shade leaves is 20% lower in C02-plants. Whereas the chlorophyll content in sun leaves stays constant during developm ent, it has increased in shade leaves by 20% at the end of the 4 months period. The content of soluble sugars is lower in C 0 2-plants compared to control plants. The difference is bigger in sun leaves than in shade leaves. The ribulose 1.5-bisphosphate carboxylase/oxygenase content almost doubles within the experimentation period, but seem s to be subject to large variations. C02-plants contain in general less ribulose 1.5-bisphosphate carboxylase/oxygenase than control plants. The content of coupling factor of photophosphorylation is 20% lower in C02-plants when compared to control plants and remains during developm ent more constant in C02-plants. The molecular structure of the photosystem II-complex undergoes under the influence o f the increased C 0 2-content a quantitative modification. The light harvesting com plex (LHCP) and the ex­ trinsic peptide with the molecular mass of 33 kDa increase in C02-plants. Gassing with S 0 2 (0.3 ppm in air) leads to a strong damage of the plants. The damaging influence is already seen after 6 days and leads to a partial leaf-shedding o f the tree. In the visually still intact remaining leaves the chlorophyll content referred to unit leaf area decreases by 63%, that of soluble sugars by 65%, the content of soluble proteins and that of Rubisco decrease by 26% and 36% respectively. The light harvesting complex and the chloro-phyll-binding peptides (43 and 47 kD a) increase whereas the extrinsic peptides decrease. It looks as if the simultaneous application of S 0 2 (0.3 ppm) and increased C 0 2 (700 ppm) releaves the damaging effect of S 0 2. Plant growth does not exhibit a difference in comparison to control plants. Soluble proteins and chlorophyll increase by 27% and 33% and the ribulose 1.5-bisphosphate carboxylase/oxygenase content as well as that o f soluble sugars increases by 18 respectively 14%. The peptide composition o f photosystem II shows a quantitative modification. The LHCP increases and the chlorophyll-binding peptides and the peptides with a molecular mass smaller than 24 kDa are reduced. The quantity of extrinsic peptides appears unchanged. Ribulose 1,5-bisphosphate carboxylase/oxygenase and the CFj-complex o f Aleurites are immunochemically only partially identical to the corresponding enzymes of Nicotiana taba­ cum as demonstrated by tandem-cross-immune electrophoresis. 
  Reference    Z. Naturforsch. 51c, 441 (1996); received May 7/May 16 1996 
  Published    1996 
  Keywords    Aleurites montana, CO2, SO2, S02-D am age, LHCP-Complex, Photosystem II-Complex, C F r Complex 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0441.pdf 
 Identifier    ZNC-1996-51c-0441 
 Volume    51