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1983 (1)
1978 (1)
1976 (1)
1Author    G. Kulandaivelu, D. 0. HallRequires cookie*
 Title    Stabilization of the Photosynthetic Activities of Isolated Spinach Chloroplasts during Prolonged Ageing  
 Abstract    Isolated spinach chloroplasts (type A complete) were used to study the changes in the pnoto-chemical activities upon storage in order to establish optimum conditions for prolonged storage. Chloroplasts stored at — 5 °C were found to retain over 70% of their photosynthetic electron transport from H20 even after 5 days. Increases in the level of state 2 electron transport (due to uncoupling) with concomitant loss of state 3 phosphorylation activity was observed during the initial period of ageing. Addition of 1% bovine serum albumin decreased the level of uncoupling and maintained the phosphorylation activity for a longer period. Chloroplasts stored at 77 °K main­ tained their phosphorylation capacity for a period of 10 days even after repeated freezing and thawing. Photosystem I activity was found to be more stable over the whole ageing period of 15 days. Loss of plastoquinone may be responsible for the decrease in electron transport between photosystems II and I. 
  Reference    (Z. Naturforsch. 31c, 452 [1976]; received August 22 1975/March 11 1976) 
  Published    1976 
  Keywords    Ageing, Stabilization, Photosynthetic Activities, Chloroplasts 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0452.pdf 
 Identifier    ZNC-1976-31c-0452 
 Volume    31 
2Author    HainfriedE A Schenk, M. Argarete, Neu-M ÜllerRequires cookie*
 Title    Der Einfluß von Cycloheximid und Chloramphenicol auf die Biosynthese der Photosynthese-Pigmente in Cyanophora paradoxa Korsch I. Photosynthetische Sauerstoffproduktion Photosynthetic O xygen Evolution  
 Abstract    The Influence o f C yclohexim ide and C hloram phenicol on the B iosynthesis o f the Photosynthetic P igm ents in Cyanophora paradoxa I. It is not clear whether an endocytobiont is able to develop during a long evolution tim e only to an organelle like cell structure (defect m utant) with a high degree of m etabolic dependency or also to a perfect cell organelle with genetical dependency to the host nucleus (intercom partm ental translocation of proteins an d /o r gene transfer) in the sense of the Serial Endocytobiosis Hypothesis (SEH). Cyanocyta korschokoffiana, the endocyanelle of C. paradoxa, seems to be a very suitable object to answer this question. Therefore we have studied the influence o f the translation inhibitors cycloheximide (CHI) and chloramphenicol (CA) on the biosynthetic behaviour of this cyanobacterial endocytobiont regarding the photosynthetic oxygen during 20 h developing and incubation time. After dilution with fresh culture m edium the oxygen evolution of the control culture remains nearly constant, if it is related to the phycocyanin content, whereas it increases slowly to a constant level relating to the chlorophyll content (nearly 140-150% o f the starting activity). CHI and CA show a rapid effect on the oxygen evolution. At the first 8 -10 h the oxygen evolution of cultures treated with CA are hardly dim inished and rem ain m ore or less constant with regard to the starting activity, but that of cultures treated with CHI decreases strongly. After 24 h the CHI treated flagellate is photosynthetically inactive and far-reaching destroyed (microscopic observations), indicating that CHI has beside the inhibition effects on protein synthesis also secondary effects on the stability o f m em branes o f C. paradoxa. Der apochlorotische Flagellat Cyanophora 
  Reference    Z. Naturforsch. 38c, 978—983 (1983); received July 19 1983 
  Published    1983 
  Keywords    Cyanophora paradoxa, Photosynthetic Activity, Oxygen Evolution, Chlorophyll, Phycocyanin 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0978.pdf 
 Identifier    ZNC-1983-38c-0978 
 Volume    38 
3Author    K.-P HeiseRequires cookie*
 Title    Variation in Chloroplast Lipid Content and its Correlation to Photosynthetic Activities  
 Abstract    During illum ination infiltrated spinach leaf sections show after a short log a linearly increasing 14C-incorporation into m em brane lipids from H 14C 0 3—. Isolated intact chloroplasts show this increase only for the neutral lipid fraction (N L). Glyco-and phospholipid accum ulation declines in the light. In chloroplast suspensions the increase in content and also in its 14C-incorporation rate of the NL fraction is accom panied 1. by a proportional decrease of CO*-fixation capacity, 2. by a decrease of the P /2 e-quotient. From 14C-incorporation kinetics, this increase of neutral lipids in plastid suspensions even after short illum ination periods appears to be a consequence of an inhibited glycolipid — and phospho­ lipid synthesis from diglycerides. 
  Reference    Z. Naturforsch. 33c, 685 (1978); received June 27 1978 
  Published    1978 
  Keywords    14C -Incorporation, Lipids, Chloroplasts, Photosynthetic Activities 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0685.pdf 
 Identifier    ZNC-1978-33c-0685 
 Volume    33