| | 2 | Author
| Daniela Vedaldi, Sergio Caffieri, Giorgia Miolo, Francesco Dall 'acqua, Paola Arslan | Requires cookie* | | | Title
| Dark and Photohemolysis of Erythrocytes by Furocoumarins  | | | | Abstract
| It has been shown that various furocoumarins are able to cause dark hemolysis in red blood cells (RBC). However, this effect is evident only at relatively high furocoumarin concentrations (4.6 x 10~ 4 M) -much higher than those used in photosensitization experiments or photo-chemotherapeutic treatments. Among the various furocoumarins examined in this study, only psoralen (Ps) and 3-carbethoxy-psoralen (3-CPs) showed strong photohemolytic effects, while the other compounds revealed little or no activity. This fact indicates that Ps and 3-CPs are able to induce selective damage to the cell membrane of RBC. By pre-irradiating furocoumarin in ethanol or isotonic saline solutions and adding the irradiated solutions to a RBC suspension, hemolysis was observed in various compounds. The products of photolysis which form during pre-irradiation may be responsible, in terms of hemolysis, for toxic effects on RBC. | | | |
Reference
| Z. Naturforsch. 43c, 888—892 (1988); received August 2 1988 | | | |
Published
| 1988 | | | |
Keywords
| Furocoumarins, Hemolysis, Photosensitization, Cell Membrane Damage, Photochemotherapy | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/43/ZNC-1988-43c-0888.pdf | | | | Identifier
| ZNC-1988-43c-0888 | | | | Volume
| 43 | |
| 3 | Author
| S. G. Mauriño, M. A. Vargas, C. Echevarría, P.J A Paricio, J.M M Aldonado | Requires cookie* | | | Title
| Red-Light Effects Sensitized by Methylene Blue on Nitrate Reductase from Spinach (Spinacia oleracea L.) Leaves | | | | Abstract
| Nitrate reductase from spinach (Spinacia oleracea L.) leaves, w hich had been inactivated in vitro by incubation with N A D H and cyanide, was fully reactivated in m inutes when irradiated in anaerobic conditions with red light in the presence o f m ethylene blue. Both the rate and the extent o f reactivation increased with light intensity (6 to 100 W -m " 2) and dye concentration (1 to 10 jiM). On the contrary, photoreactivation was com pletely abolished when N A D H or ethylenediaminetetra-acetic acid were present during irradiation. W e propose that m ethylene blue, when photo excited, exhibits a redox potential positive enough to reoxidise the CN~-re-duced molybdenum complex settled in the inactive enzym e, thus causing its reactivation. On the other hand, prolonged irradiation o f nitrate reductase, under air and in the presence o f methylene blue, promoted an oxygen-dependent irreversible inactivation o f the two partial activities o f the enzyme. This inactivation was m arkedly enhanced in 77% deuterated water and greatly prevented by azide, which indicates that singlet oxygen is the species primarily involved in the photooxidative inactivation o f the enzym e. | | | |
Reference
| Z. Naturforsch. 39c, 1079—1084 (1984); received July 27 1984 | | | |
Published
| 1984 | | | |
Keywords
| Methylene Blue, Nitrate Reductase, Photosensitization, Singlet O xygen, Spinacia | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/39/ZNC-1984-39c-1079.pdf | | | | Identifier
| ZNC-1984-39c-1079 | | | | Volume
| 39 | |
|
