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1997 (1)
1Author    Britta Förster3, PeterB. Heifetz0, Anita Lardansd, JohnE. Boyntona, NicholasW. GillhambRequires cookie*
 Title    Herbicide Resistance and Growth of D1 Ala25i Mutants in Chlamydomonas  
 Abstract    We elucidated the effects of substituting seven amino acids for Ala at residue 251 of the Chlam ydom onas reinhardtii D1 protein on herbicide resistance and photoautotrophic growth. A la25i has been suggested to play a key role in the structural integrity and function of the stromal loop between transmembrane helices IV and V of D1 and has previously been shown to affect resistance to "classical" PSII specific herbicides. Sensitive and rapid microtiter assays were employed to compare herbicide resistance and photoautotrophic growth in the various mutants. Substitution of Ala25i by Ile, Leu or Val conferred resistance to the PSII herbicides atrazine, bromacil and metribuzin but not to DCMU, and impaired photoautotrophic growth in high and low light. Compared to an otherwise isogenic wildtype strain, the lie and Val mutants exhibited nearly identical levels of herbicide resistance and reduced growth while the Leu mutant had even slower growth and higher levels of herbicide resistance. In contrast Cys, Pro, Ser and Gly mutants were phenotypically indistinguishable from wildtype in terms of herbicide sensitivity and photoautotrophic doubling times. Collectively the seven A la251 mutations differed markedly from an Ala mutant (dr-1) at the well characterized Ser264 D1 residue in terms of herbicide resistance and photoautotrophic growth. 
  Reference    Z. Naturforsch. 52c, 654—664 (1997); received June ll/Ju ly 2 1997 
  Published    1997 
  Keywords    D1 Protein, Site-Directed Chloroplast Mutations, Herbicide Resistance, Photoautotrophic Growth, Microtiter Assay 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0654.pdf 
 Identifier    ZNC-1997-52c-0654 
 Volume    52