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'Photoaffinity Labelling' in keywords Facet   Publication Year 1990  [X]
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1Author    HelenG M Cfadden, JohnN. PhillipsRequires cookie*
 Title    Synthesis and Use of Radiolabelled Cyanoacrylate Probes of the Photosystem II Herbicide Binding Site  
 Abstract    Cyanoacrylates are potent inhibitors o f photosynthetic electron transport (PET) and are po­ tentially useful probes o f the photosystem II herbicide binding site. A series o f cyanoacrylates was synthesized and the Hill inhibition activities evaluated in order to select com pounds suita­ ble for radioactive synthesis. A cyanoacrylate, 2-(2-nitrophenoxy)ethyl 3-benzylamino-2-cyano-2-pentenoate, was found to displace diuron from the photosystem II herbicide binding site. For this compound the dissociation constant o f the inhibitor/binding site complex was found to be 2 x 10"8 M with an active site concentration o f 2 nm ol/m g chlorophyll. In a similar system the corresponding figures for diuron were 1.2 x 10"8 m and 1.3 nm ol/m g chlorophyll. Photoaffinity labelling o f 1% II thylakoid proteins with 2-(2-azidophenoxy)ethyl 3-[7-l4C]-benzylam ino-2-cyano-2-pentenoate showed weak binding in the 32 and 28 kD mass regions, consistent with binding to the D, peptide. 
  Reference    Z. Naturforsch. 45c, 196—202 (1990); received September 20/N ovem ber 10 1989 
  Published    1990 
  Keywords    Cyanoacrylate, Photoaffinity Labelling, Photosystem II, Com petitive Inhibition, Diuron 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0196.pdf 
 Identifier    ZNC-1990-45c-0196 
 Volume    45