| | 1 | Author
| R. Egina, H. Aase, M. Onika, U. N. Th, U. Tu Rier, A. Lfons, R. Adunz, G. Eorg, H. Schm | Requires cookie* | | | Title
| Determination of Glycolipids, Sulfolipid and Phospholipids in the Thylakoid Membrane  | | | | Abstract
| D eterm ination of binding o f antibodies to lipids onto the surface o f the thylakoid mem brane, before and after the removal of the CF,-complex with sodium bromide, showed that in the immediate vicinity of CF, sulfolipid and monogalactolipid occur in higher concentration and are therefore arranged in domains. The m olar ratio of the CF,-complex to glycolipids was determined in Nicotiana tabacum chloroplasts o f different structure. Thus, in the chlorophyll-deficient tobacco m utants N. tabacum Su/su and Su/su var. Aurea, the m olar ratio o f C F ,/ m onogalactolipid is the same and found to be 1:570. The structure o f the lamellar system in these m utants is characterized by a higher ratio o f strom a lamellae to grana stacks when com pared to the green wild type. In the wild type the ratio CF,/m onogalactolipid is 30 per cent larger (1 :740). In contrast to this the m olar ratio CF,/sulfolipid and CF,/digalactolipid is the same in the wild type and the Su/su mutant, whereas these ratios are twice as high in the yellow m utant Nicotiana tabacum Su/su var. Aurea. The binding of glycolipids and phospholipids onto the subunits o f CF, from Spinacia olera-cea was determined in the Western blot procedure by using monospecific antisera. These ex periments lead to the result that the two large subunits (a and ß) are m arked by antisera to monogalactosyldiglyceride, digalactosyldiglyceride and sulfoquinovosyldiglyceride. The anti sera to phospholipids react differently: whereas the antiserum to phosphatidylinositol only reacts with the a-subunit, the antiserum to phosphatidylcholine and that to phosphatidylgly-cerol react just as the antisera to glycolipids with both large subunits. It is observed that the antiserum to monogalactolipid occasionally m arks the y-subunit. This might mean that the glycolipids and the respective phospholipids are tightly bound onto the reacting a-and ß-subunits of the CF,-complex. Incubation of the subunit CF, with lipase from Rhizopus arrhi-zus and with phospholipase C from Chlostridium perfringens after their transfer to the nitrocel lulose membrane abolishes the positive reaction of the peptides with the antisera to glycolipids and phospholipids. | | | |
Reference
| Z. Naturforsch. 48c, 623—631 (1993); received May 17 1993 | | | |
Published
| 1993 | | | |
Keywords
| CF, -Subunits, Glycolipids, Phospholipids, Antibodies, Lipid-Binding | | | |
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| default:Reihe_C/48/ZNC-1993-48c-0623.pdf | | | | Identifier
| ZNC-1993-48c-0623 | | | | Volume
| 48 | |
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