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'Phosphinothricin A acetyltransferase PAT' in keywords
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1995 (1)
1Author    Josef Vinnemeier, Wolfgang Dröge-Laser, ElfriedeK. Pistorius, Inge BroerRequires cookie*
 Title    Purification and Partial Characterization of the Streptomyces viridochromogenes TÜ494 Phosphinothricin-N-acetyltransferase Mediating Resistance to the Herbicide Phosphinothricin in Transgenic Plants  
 Abstract    A purification scheme for the enzyme phosphinothricin-N-acetyltransferase (PAT) origi­ nating from Streptomyces viridochrom ogenes {pat-gene product from Streptom yces virido­ chromogenes) and mediating herbicide resistance to transgenic plants was developed. The enzyme was isolated from a transformed and overproducing Escherichia coli strain. With a combination of ammonium sulfate fractionation, chromatography on D EAE-Sephadex A50-, Phenylsepharose-, Hydroxylapatite-and FPLC-Superose 12-columns it was possible to obtain PAT which was at least 90 % homogeneous on the basis of SDS-PAGE. The properties of the isolated PAT were compared with the properties of PAT from S. hygroscopicus (bar-gene product from S. hygroscopicus) previously isolated and characterisized by Botterman, J., Gossele, V., Thoen, C., Lauwereys, M. (1991), G ene 102, 33-37. Differences were observed in the molecular masses of the two native enzymes (PAT from S. viridochrom ogenes being a dimer of 40 kD and PAT from S. hygroscopicus being a m onomer of 21 kD), and in the tem perature sensitivity of the two enzymes (the PAT from S. viridochrom ogenes being slightly more tem perature stable than PAT from S. hygroscopicus). However, since the pat and the bar-gene are to 85 % homologous, substantial similarities exist between the two enzymes especially in the kinetic values and the substrate specificity. The isolated S. virido­ chromogenes PAT did not acetylate putative substrates present in the plant cell. Antibodies were raised against the isolated protein. This antiserum was able to detect PAT in transgenic plants and therefore is suitable to analyse the fate of the protein in such plants under various stress conditions. 
  Reference    Z. Naturforsch. 50c, 796—805 (1995); received August 16 1995 
  Published    1995 
  Keywords    Biosafety, Herbicide Resistance, Nicotiana tabacum, Phosphinothricin-A'-acetyltransferase (PAT), Transgenic Plants 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0796.pdf 
 Identifier    ZNC-1995-50c-0796 
 Volume    50